Mutagenicities of 8-Hydroxyguanine and 2-Hydroxyadenine Produced by Reactive Oxygen Species (Biological and Pharmaceutical Aspects of Nucleic Acids Chemistry)
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概要
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Oligodeoxyribonucleotides containing 8-hydroxyguanine and 2-hydroxyadenine, purine lesions produced in cells by reactive oxygen species, were synthesized and inserted into vector DNAs to introduce each lesion at a predetermined site. The manipulated DNAs were transfected into living cells, and the mutants induced by each DNA lesion were collected and analyzed. In addition, the mutations induced by damaged DNA precursors with the two oxidized purine bases were studied by the use of chemically synthesized nucleoside triphosphates. In this review article, the author summarizes the mutagenic potentials of the two oxidized purine bases, by focusing on experiments examined by the author and his collaborators.
- 公益社団法人日本薬学会の論文
- 2004-04-01
著者
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KAMIYA Hiroyuki
Graduate School of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Graduate School Of Pharmaceutical Sciences Hokkaido University
関連論文
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- 2-Hydroxyadenine in DNA is a Very Poor Substrate of the Escherichia coli MutY Protein
- Silencing of Exogenous DNA in Cultured Cells
- Effect of Methylated Adenine in Plasmid DNA on Transgene Expression in Mice(Biopharmacy)
- Induction of Transition and Transversion Mutations during Random Mutagenesis PCR by the Addition of 2-Hydroxy-dATP (Biochemistry/Molecular Biology)
- Correction of Frameshift Mutations with Single-Stranded and Double-Stranded DNA Fragments Prepared from Phagemid/Plasmid DNAs(Miscellaneous)
- Mutagenicity of 5-Formylcytosine, an Oxidation Product of 5-Methylcytosine, in DNA in Mammalian Cells
- Mutagenicities of 8-Hydroxyguanine and 2-Hydroxyadenine Produced by Reactive Oxygen Species (Biological and Pharmaceutical Aspects of Nucleic Acids Chemistry)
- Foreword (Biological and Pharmaceutical Aspects of Nucleic Acids Chemistry)
- DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7, 8-dihydroguanine(8-hydroxyguanine)Paired with Adenine