Comparison of Oxidation Products from DNA Components by γ-Irradiation and Fenton-Type Reactions

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概要

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• The four 2'-deoxyribonucleosides were γ-irradiated or were aerobically treated with Fenton-type-reagents, Fe(II)-EDTA or a renal carcinogen Fe(II)-nitrilotriacetic acid (NTA) under the neutral conditions. The reaction mixtures were immediately analyzed by reverse-phase HPLC. Major products detected were 2-hydroxydeoxyadenosine (2-OR-dA), 8, 5'-cyclodeoxyadenosine (cyclo-dA), 8-hydroxydeoxyadenosine (8-OH-dA), 5-formyldeoxyuridine (5-CHO-dU), 5-hydroxydeoxycytidine (5-OH-dC), 8-hydroxydeoxyguanosine (8-OH-dG), 8, 5'-cyclodeoxyguanosine (cyclo-dG), and glyoxal and its adduct with dG. Ratio of these oxidized products were dramatically changed depending upon the agents used. For example, 2-OH-dA was a modified nucleoside produced most efficiently by Fe(II)-EDTA, while 5-CHO-dU and 5-OH-dC were the major products by the Fe(II)-NTA treatment and y-irradiation, respectively. Glyoxal itself was estimated to be produced most frequently (13 folds of 8-OH-dG) when treated with Fe(II)-EDTA, but its formation was not detected by the treatment with Fe(II)-NTA or by γ-irradiation. 8-OH-dA was not produced by Fe-EDTA or Fe-NTA but was produced by γ-irradiation. In contrast, 2-OH-dA was not produced by γ-irradiation. These results suggest that triphosphates of 2-OH-dA, cyclo-dA, 8-OH-dA, cyclo-dG, 5-CHO-dU, 5-OH-dC, and glyoxal-dG as well as 8-OH-dG may be produced in cells with different ratio by various types of oxidative stress and involved in mutagenesis and carcinogenesis.

• 日本放射線影響学会の論文

著者

• Kamiya Hiroyuki

  Department of Surgery (I), Kanazawa University School of Medicine

• Kasai Hiroshi

  Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occ

• MURATA-KAMIYA Naoko

  Inst. Indust. Ecolog. Sci., Univ. Occup. Environ. Hith.

• KAJI HIDESUKE

  College of Nursing Art and Culture 13-71

• Kamiya Hiroyuki

  産業医科大学産業生態科学研究所

• Murata-kamiya Naoko

  Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.

• MURATA-KAMIYA NAOKO

  Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University

• MURAOKA MASAKO

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• KAJI HIROSHI

  Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University

• Kasai H

  Department Of Environmental Oncology University Of Occupational And Environmental Health

• Kamiya Hiroyuki

  Department Of Surgery (i) Kanazawa University School Of Medicine

• Kasai Hiroshi

  Department Of Ennvironmental Oncology Institute Of Industrial Ecological Sciences

• Murata‐kamiya N

  Department Of Health Policy And Management Institute Of Industrial Ecological Sciences University Of

• Muraoka Masako

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Kasai H

  Division Of Diabetes Metabolism And Endocrinology Department Of Internal Medicine Kobe University Gr

• Muraoka M

  Japan Women's Univ. Tokyo Jpn

• Kaji Hiroshi

  Department Of Health Policy And Management Institute Of Industrial Ecological Sciences University Of

• Kamiya Hiroyuki

  Department Of Microbiology Tokyo Medical University

• Kamiya Hiroyuki

  Department of Cardiovascular Surgery and Research Group for Experimental Surgery, Heinrich Heine University, Medical Faculty

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