Analysis of 8-Hydroxyguanine (8-OH-Gua) Released from DNA by the Formamidopyrimidine DNA Glycosylase (Fpg) Protein : A Reliable Method to Estimate Cellular Oxidative Stress
スポンサーリンク
概要
- 論文の詳細を見る
To improve the analyses of a form of oxidative DNA damage, 8-hydroxyguanine (8-OH-Gua), we treated isolated DNA with formamidopyrimidine DNA glycosylase (Fpg) and analyzed the released 8-OH-Gua by using a high-performance liquid chromatography system equipped with an electrochemical detector (HPLC-ECD). The human lung carcinoma cells (A549) and human keratinocyte (HaCaT) were irradiated with γ-rays. After the isolated DNA was treated with the Fpg protein, we analyzed the released 8-OH-Gua by using an HPLC-ECD. With this method, the background level of 8-OH-Gua in DNA from human lung carcinoma cells was determined to be 3.4 residues per 10^7 guanine (Gua). A similar background level of 8-OH-Gua (3.1 residues per 10^7 Gua) was also detected in human keratinocyte DNA with this method. These background 8-OH-Gua levels in cellular DNA are comparable to that obtained previously by an analysis of 8-OH-dGMP after nuclease P1 digestion of cellular DNA (4.3 residues per 10^7 dCMP). A dosedependent increase of 8-OH-Gua (0.17/10^7 Gua/Gy) was observed after cells were irradiated with γ-rays. Twenty hours after γ-irradiation with 60 Gy, 75% of the 8-OH-Gua produced in keratinocyte DNA was repaired. With our new analysis method, it is possible to detect the small changes in the 8-OH-Gua levels in cellular DNA induced by various environmental factors.
- 日本放射線影響学会の論文
- 2004-10-10
著者
-
Kasai Hiroshi
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occ
-
TOKURA Yoshiki
Department of Dermatology, Hamamatsu University School of Medicine
-
ORIMO Hiroshi
Department of Dermatology University of Occupational and Environmental Health
-
Mei Nan
Division Of Genetic And Reproductive Toxicology National Center For Toxicological Research Fda
-
Kasai Hiroshi
Department Of Environmental Oncology Institute Of Industrial Ecological Sciences
-
Kasai Hiroshi
Department Of Ennvironmental Oncology Institute Of Industrial Ecological Sciences
-
Tokura Yoshiki
Department Of Dermatology Hamamatsu University School Of Medicine
-
BOITEUX Serge
Commissariat al'Energie Atomique (CEA), Direction des Sciences du Vivant (DSV), Departement de Radio
-
Boiteux Serge
Commissariat Al'energie Atomique (cea) Direction Des Sciences Du Vivant (dsv) Departement De Ra
-
Orimo Hiroshi
Department of Dermatology, University of Occupational and Environmental Health
関連論文
- Establishment of murine model of allergic photocontact dermatitis to ketoprofen and characterization of pathogenic T cells
- Analysis of 8-OH-dG and 8-OH-Gua as Biomarkers of Oxidative Stress
- S-08 4-Oxo-2-hexenal, a mutagen formed by ω-3 fat peroxidation : occurrence, detection and adduct formation(ACEM Symposium II:Mutagens and anti-mutagens in Asian foods)
- Increase of Urinary 8-OH-dG Levels after Administration of a Vitamin-deficient Diet and a Sweet Beverage
- Accurate Measurement of 8-OH-dG and 8-OH-Gua in Mouse DNA, Urine and Serum : Effects of X-ray Irradiation
- Infrequent Mutations of the hOGG1 Gene, That Is Involved in the Excision of 8-Hydroxyguanine in Damaged DNA, in Human Gastric Cancer
- Lung Cancer Patients Have Increased 8-Hydroxydeoxyguanosine Levels in Peripheral Lung Tissue DNA
- Soluble form of vascular cell adhesion molecule-1 in systemic lupus erythematosus and discoid lupus erythematosus
- A Leg Ulcer due to Protein C Deficiency : Successful Treatment with Split Thickness Skin Graft after Protein C Supplementation
- Fragmentation of the DNA Repair Enzyme, OGG1, in Mouse Nonparenchymal Liver Cells by Arsenic Compounds
- Detection of a mouse OGG1 fragment during caspase-dependent apoptosis : Oxidative DNA damage and apoptosis
- Sequence Specificity of the 8-Hydroxyguanine Repair Activity in Rat Organs
- Increase in 8-Hydroxyguanine and Its Repair Activity in the Esophagi of Rats Given Long-term Ethanol and Nutrition-deficient Diet
- Analyses of Oxidative DNA Damage and Its Repair Activity in the Livers of 3'-Methyl-4-dimethylaminoazobenzene-treated Rodents
- Inhibition of Nucleolar Function and Morphological Change by Adriamycin Associated with Heat Shock Protein 70 Accumulation
- Body mass index and oxidative DNA damage : A longitudinal study
- Comparison of Oxidation Products from DNA Components by γ-Irradiation and Fenton-Type Reactions
- P058 A multi-center and multi-biomarker study of oxidative stress in adolescents exposed to AFB1
- O-15 A multi-center and multi-biomarker study of oxidative stress in adolescents exposed to AFB1(ACEM Oral Presentation III)
- A case of laryngeal carcinoma in a young adult with dyskeratosis congenita
- Acne Fulminans Coexisting with Pyoderma Gangrenosum-like Eruptions and Posterior Scleritis
- Psychosocial Factors as a Potential Trigger of Oxidative DNA Damage in Human Leukocytes
- Hypersensitivity to Mosquito Bites Is Not an Allergic Disease, But an Epstein-Barr Virus-Associated Lymphoproliferative Disease
- Hypersensitivity to Mosquito Bites Conceals Clonal Lymphoproliferation of Epstein-Barr Viral DNA-positive Natural Killer Cells
- Effect of age, smoking and other lifestyle factors on urinary 7-methylguanine and 8-hydroxydeoxyguanosine
- Life Style and Urinary 8-Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage : Effects of Exercise, Working Conditions, Meat Intake, Body Mass Index, and Smoking
- Alterations of serum Th1 and Th2 chemokines by combination therapy of interferon-γ and narrowband UVB in patients with mycosis fungoides
- Alterations of immune functions in barrier disrupted skin by UVB irradiation
- Lupus Erythematosus Associated with C1 Inhibitor Deficiency
- Dominant expression of CXCR3 is associated with induced expression of IP-10 at hapten-challenged sites of murine contact hypersensitivity : a possible role for interferon-γ-producing CD8^+ T cells in IP-10 expression
- Unique therapeutic effects of the Japanese-Chinese herbal medicine, Sairei-to, on Th1/Th2 cytokines balance of the autoimmunity of MRL/lpr mice
- Lymphocyte stimulation test with drug-photomodified cells in patients with quinolone photosensitivity
- Roxithromycin down-modulates antigen-presenting and interleukin-1β-producing abilities of murine Langerhans cells
- Membranous and Soluble Forms of Fas Antigen in Cutaneous Lupus Erythematosus
- Systemic Administration of Hochu-ekki-to (Bu-Zhong-Yi-Qi-Tang), a Japanese-Chinese Herbal Medicine, Maintains Interferon-γ Production by Peripheral Blood Mononuclear Cells in Patients with Mycosis Fungoides
- Evaluation of Soluble Cell Adhesion Molecules in Atopic Dermatitis
- Selective Expansions of T cells Expressing Vβ8 and Vβ13 in Skin Lesions of Patients with Chronic Cutaneous Lupus Erythematosus
- The Macrolide Antibiotic, Roxithromycin Suppresses IFN-γ-Mediated Immunological Functions of Cultured Normal Human Keratinocytes
- The role of low-density lipoprotein receptors in sensitivity to killing by Photofrin-mediated photodynamic therapy in cultured human tumor cell lines
- Analysis of 8-Hydroxyguanine (8-OH-Gua) Released from DNA by the Formamidopyrimidine DNA Glycosylase (Fpg) Protein : A Reliable Method to Estimate Cellular Oxidative Stress
- Adult T-cell leukemia/lymphoma cells from blood and skin tumors express cytotoxic T lymphocyte-associated antigen-4 and Foxp3 but lack suppressor activity toward autologous CD8^+ T cells
- The Stability of the Oxidative Stress Marker, Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), when Stored at Room Temperature
- P-142 Simultaneous analysis of various oxidative DNA adducts in human tissues using LC-MS/MS(Poster Session)
- Effects of oral antibiotic roxithromycin on quality of life in acne patients
- Message from the President of the Conference
- A New AutomatedMethod to Analyze Urinary 8-Hydroxydeoxyguanosine by a High-performance Liquid Chromatography-Electrochemical Detector System
- Lupus Erythematosus Profundus with Unusual Skin Manifestation : Subcutaneous Nodules Coexisting with Eyelid Plaques
- Photohapten TCSA painting plus UVA irradiation of murine skin augments the expression of MHC class II molecules and CD86 on Langerhans cells
- T cell populations propagating in the peripheral blood of patients with drug eruptions
- The Various Effects of Four H1-Antagonists on Serum Substance P Levels in Patients with Atopic Dermatitis
- A Nevocellular Nevus Consisting mostly of Nevic Corpuscles
- Treatment with IFN-γ increases serum levels of Th1 chemokines and decreases those of Th2 chemokines in patients with mycosis fungoides
- A Japanese Case of Typical Epstein-Barr Virus Non- Associated Subcutaneous Panniculitis-Like T- Cell Lymphoma
- Evaluation of photoallergic potential of chemicals using THP-1 cells
- Formation of 8-hydroxy-2'-deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A
- Detection of Genotoxic Nucleosides, 8-Hydroxydeoxyguanosine, 8-Hydroxyguanosine and Free Base 8-Hydroxyguanine, in Fish Food Products
- Induction of eosinophil-infiltrating drug photoallergy in mice
- Folliculotropic mycosis fungoides presenting as papuloerythroderma
- Occupational and lifestyle factors and urinary 8-hydroxydeoxyguanosine
- Food-dependent anaphylaxis with serum IgE immunoreactive to dairy products containing high-molecular-weight proteins
- Antihistaminic drug olopatadine downmodulates CCL17/TARC production by keratinocytes and Langerhans cells
- Mixed tumor of the skin arising on the auricle
- Skin application of ketoprofen systemically suppresses contact hypersensitivity by inducing CD4^+ CD25^+ regulatory T cells
- Agaricus blazei Murill augments keratinocyte production of bioactive cytokines capable of promoting antigen-presenting capacity
- Fexofenadine downmodulates antigen-presenting ability of murine epidermal Langerhans cells
- Stimulation of Langerhans cells with ketoprofen plus UVA in murine photocontact dermatitis to ketoprofen
- Inhibitory effects of epinastine on chemokine production and MHC class II/CD54 expression in keratinocytes
- Nadifloxacin downmodulates antigen-presenting functions of epidermal Langerhans cells and keratinocytes
- DNA Methylation at the C-5 Position of Cytosine by a Methyl Radical : A Link between Environmental Agents and Epigenetic Change
- Evaluation of toxicity and Stat3 activation induced by hydrogen peroxide exposure to the skin in healthy individuals
- Drug-induced papuloerythroderma of Ofuji : Comment
- Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA
- Expression of T-cell cytokines in challenged skin of murine allergic contact photosensitivity : low responsiveness is associated with induction of Th2 cytokines
- Quinolone photoallergy : Photosensitivity dermatitis induced by systemic administration of photohaptenic drugs
- Giant Suspensory Lipoma with Calcification
- Modulation of cytokine production by 8-methoxypsoralen and UVA
- Complete remission of metastatic malignant melanoma after surgery in association with development of systemic vitiligo
- Primary cutaneous amyloidosis of the auricular concha : Case report and review of published work
- Preferential downmodulation of certain chemokines by fexofenadine in human keratinocytes
- Reactive Oxygen Species (ROS) and Exercise : 2SB5:Physical Exercise and Oxidative DNA Damage
- Photocontact dermatitis and chloracne : two major occupational and environmental skin diseases induced by different actions of halogenated chemicals
- Extrinsic and intrinsic types of atopic dermatitis
- Greeting from the President of the Japanese Society for Investigative Dermatology (JSID) : On achieving internationalization and educating young researchers
- Angiolymphoid hyperplasia with eosinophilia on the leg successfully treated with T-helper cell 2 cytokine inhibitor suplatast tosilate
- Methionine Sulfoxide Stimulates Hepatocarcinogenesis in Non-alcoholic Steatohepatitis (NASH) Mouse : Possible Role of Free Radical-mediated DNA Methylation
- Cutaneous pseudolymphoma due to benidipine hydrochloride with massive infiltration of eosinophils
- Modulation of semaphorin 3A expression by calcium concentration and histamine in human keratinocytes and fibroblasts
- Epithelial-mesenchymal transition in the skin
- E1-3 Drug eruptions associated with telaprevir(English Session 1)
- Detection of a smaller, 32-kDa 8-oxoguanine DNA glycosylase 1 in 3'-methyl-4-dimethylaminoazobenzene-treated mouse liver
- Efficacy of combined topical treatment of acne vulgaris with adapalene and nadifloxacin : A randomized study
- Molecular mapping of lymph node metastases by real-time reverse transcription polymerase chain reaction in two melanoma patients
- Analyses of 8-Methyldeoxyadenosine and 8-Methyldeoxyguanosine as Markers of Free Radical-mediated DNA Methylation in Mouse
- Generation and Threshold Level of 8-OHdG as Oxidative DNA Damage Elicited by Low Dose Ionizing Radiation
- Analysis of 8-OH-dG and 8-OH-Gua as Biomarkers of Oxidative Stress