A New AutomatedMethod to Analyze Urinary 8-Hydroxydeoxyguanosine by a High-performance Liquid Chromatography-Electrochemical Detector System
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概要
- 論文の詳細を見る
A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OHdG fraction in the first HPLC is precisely and automatically collected based on the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.
- 日本放射線影響学会の論文
著者
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Kasai Hiroshi
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occ
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Kasai Hiroshi
Department Of Ennvironmental Oncology Institute Of Industrial Ecological Sciences
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Kasai Hiroshi
産業医科大学産業生態科学研究所 職業性腫瘍学
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