Purification and Characterization of Serine Hydroxymethyltransferase from Mitochondria of Euglena gracilis z
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概要
- 論文の詳細を見る
Mitochondrial serine hydroxymethyltransferase, L-serine: tetrahydrofolate 5,10-hydroxymethyltransferase (EC 2.1.2.1), (m-SHMT) was extracted and highly purified from Euglena gracilis z. The specific activity increased from the crude extract with 10% yield up to 580-fold through the following steps: ammonium sulfate fractionation, DEAE-cellulose column chromatography and rechromatography, and affinity chromatography with L-lysine-Sepharose 4B. The molecular weight of the purified m-SHMT was 88,000 by gel filtration through Sephadex G-200, and 44,000 by SDS-PAGE. One mol of the purified enzyme contained two mol of pyridoxal 5'-phosphate (PLP), indicating that the enzyme is a dimer. Characteristics of the enzyme were examined and compared with SHMTs of other origins. The m-SHMT of Euglena gracilis z had L-threonine aldolase activity as did s-SHMT of the same origin in addition to the usual SHMT activity.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
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NAKANO Yoshihisa
Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osak
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Masuda Tomomi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
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NAKANO Yoshihisa
Graduate School of Life and Environmental Sciences of Osaka Prefecture University
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KITAOKA Shozaburo
Department of Agricultural Chemistry, University of Osaka Prefecture
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Nakano Yoshihisa
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Nakano Yoshihisa
Department Of Agriculture University Of Osaka Prefecture
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TANIGAWA Yoshinori
Department of Biochemistry and Molecular Medicine, Shimane Medical University
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SAKAMOTO Masako
Shitennoji International Buddhist University
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MASUDA Tsutomu
Shitennoji International Buddhist University
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YANAGIMOTO Yukio
Shitennoji International Buddhist University
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Kitaoka S
Univ. Osaka Prefecture Osaka Jpn
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Kitaoka Shozaburo
Department Of Agricultural Chemistry
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Masuda T
Noda Inst. Scientific Res. Chiba Jpn
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Sakamoto M
Osaka Univ. Medical School Osaka
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Tanigawa Y
Shimane Medical Univ. Izumo Jpn
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Tanigawa Yoshinori
Department Of Biochemistry And Molecular Medicine Shimane Medical University
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Nakano Yoshihisa
Department Of Agricultural Chemistry
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