Uptake and physiological function of vitamin B_<12> in a photosynthetic unicellular coccolithophorid alga, Pleurochrysis carterae(Microbiology & Fermentation Technology)

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The photosynthetic coccolithophoid alga, Pleurochrysis (Hymenomonas) carterae, could take up and accumulate exogenous vitamin B_<12>, most of which was converted into the coenzyme forms of vitamin B_<12>. Two vitamin B_<12>-dependent enzyme activities (methylmalonyl-CoA mutase, 2.6±0.4nmol/min/mg protein and methionine synthase, 85.1±38.9 pmol/min/mg protein) could be found in a cell homogenate of the vitamin B_<12>-supplemented alga. Most of the methylmalonyl-CoA mutase activity and 19.2% of the vitamin B_<12> accumulated by the algal cells were recovered in the macromolecular fractions with M_r of 150 kDa, although the remaining vitamin B_<12> was found only in free vitamin B_<12> fractions.
社団法人日本農芸化学会の論文
2002-01-23