Resveratrol Down-Regulates the Androgen Receptor at the Post-Translational Level in Prostate Cancer Cells

概要

論文の詳細を見る
Androgen receptor (AR) functions as a transcriptional factor for the development and progression of prostate cancer. Resveratrol is known to inhibit the function of AR and to repress AR expression at the transcriptional level. This study focuses on the effects of resveratrol on the AR function and the post-translational AR level. Resveratrol repressed the transcriptional activities of a mutant AR lacking the ligand-binding domain, a constitutive active form of AR, and wild-type AR in a concentration-dependent manner in human prostate cancer PC-3 cells, indicating that resveratrol does not inhibit the transcriptional activity of AR through binding to the ligand-binding domain of AR. Furthermore, the half-life of AR protein was approximately 4h in resveratrol-treated AR-positive prostate cancer LNCaP cells, compared to approximately 13h in control cells, as determined by cycloheximide chase. These results indicate that resveratrol down-regulates AR protein through a posttranslational mechanism and suggest that the inhibitory effect of resveratrol on AR function is partly attributable to a decrease in the post-translational AR level.
日本ビタミン学会の論文