Three Extracellular Chitinases in Suspension-cultured Rice Cells Elicited by N-Acetylchitooligosaccharides
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概要
- 論文の詳細を見る
In rice suspension culture, a large part (about 90% of total activity in the culture) of the chitinase activity was found in the medium. Two extracellular chitinases (which we named RCH-A and -B) were separated from the cell suspension by DEAE-cellulofine column chromatography. When cells were treated with N-acetylchitooligosaccharides (chitin oligosaccharides) for 3 days, extracellular chitinase activity increased about 3-fold over the control culture. After the treatment, another extracellular chitinase (named RCH-C) appeared in addition to increases in the levels of RCH-A and -B. Partial amino acid sequences of these enzymes indicated that RCH-A (33.5 kDa) and -B (34 kDa) were class Ib chitinases but RCH-C (27 kDa) was a class III chitinase. RCH-A and -B were capable of actively degrading water-insoluble chitin with high affinities, while RCH-C had less affinity for the substrate. However, when a water-soluble chitin derivative, 6-O-hydroxyethylchitin (glycolchitin) was used, RCH-C as well as RCH-A and -B degraded actively with a high affinity. A synergistic effect was observed when these three chitinases acted simultaneously in the hydrolysis of chitin.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
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Yamaguchi Yasuhiro
Department of Applied Biological Science, Tokyo University of Science
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INUI Hiroshi
Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osak
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Inui H
Tottori Univ. Tottori Jpn
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Inui Hiroshi
Department Of Agricultural Biochemistry And Biotechnology Tottori University:(present Address)depart
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Inui Hiroshi
Department Of Applied Biological Chemistry Osaka Prefecture University
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Inui Hiroshi
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Inui Hiroshi
Graduate School Of Life And Environmental Sciences Of Osaka Prefecture University
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Ihara Hideshi
College Of Integrated Arts And Sciences
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Ihara Hideshi
College Of Integrated Arts And Sciences Osaka Prefecture University
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YAMADA Taku
Department of Applied Chemistry, Nagoya Institute of Technology
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Yamada Taku
Department Of Agricultural Biochemistry And Biotechnology Tottori University:department Of Applied B
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HIRANO Shigehiro
Department of Agricultural Biochemistry and Biotechnology, Tottori University
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ISHIGAMI Yoshitsugu
Department of Agricultural Biochemistry and Biotechnology, Tottori University
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KAWAGUCHI Satoru
Department of Agricultural Biochemistry and Biotechnology, Tottori University
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Yamaguchi Y
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Kawaguchi Satoru
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Hirano S
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Hirano Shigehiro
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Ishigami Yoshitsugu
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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Yamaguchi Yasuhiro
Department Of Agricultural Biochemistry And Biotechnology Tottori University
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