Isolation of Physiologically Intact Chloroplasts from Euglena gracilis z
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概要
- 論文の詳細を見る
Chloroplasts were isolated from E. gracilis z grown on a vitamin B_<12> -limiting medium. The procedure is consisted of partial trysin digestion of the Euglena cells, subsequent mechanical disruption of the cells and collection of Chloroplasts by differential centrifugation. The isolated Chloroplasts fixed CO_2 at a rate of 20 μmoles of CO_2 per mg chlorophyll per hr, corresponding to one-third of the photosynthetic activity of the original cells. Linear sucrose density gradient centrifugation of the crude cell homogenate obtained by the digestive method shows that chlorophyll and most of the activity of ribulose biphosphate carboxylase cosediment as only one peak to the fraction corresponding to an equilibrium density of 1.165 g/cm^3. Based on these results we disignate the Chloroplasts isolated by the digestive method as the "intact" chloroplasts.
- 大阪府立大学の論文
- 1980-03-31
著者
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Yokota Akiho
Department Of Agricultural Chemistry University Of Osaka Prefecture
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NAKANO Yoshihisa
Graduate School of Life and Environmental Sciences of Osaka Prefecture University
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Nakano Yoshihisa
Nutrition Laboratory College Of Agriculture
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KITAOKA Shozaburo
Nutrition Laboratory, College of Agricurture
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Kitaoka S
Univ. Osaka Prefecture Osaka Jpn
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Kitaoka Shozaburo
Nutrition Laboratory College Of Agriculture
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Kitaoka S
Univ. Osaka Prefecture Sakai Jpn
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SHIGEOKA Shigeru
Nutrition Laboratory, College of Agriculture
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YOKOTA Akiho
Nutrition Laboratory, College of Agriculture
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