Regulation of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Activation by Inorganic Phosphate through Stimulating the Binding of the Activator CO_2 to the Activation Sites : PROTEINS, ENZYMES AND METABOLISM
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概要
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The mechanism of the regulation of the activation of ribulose 1,5-bisphosphate carboxylase/ oxygenase (RuBisCO) by inorganic phosphate (P_i) in the presence of limiting concentrations of CO_2 was explored. The activation state of RuBisCO increased sigmoidally following a biphasic kinetics against the concentration of P_i in the activation mixture with an intermediary plateau at 2 to 3 mM P_i When the enzyme was activated for 30 min. The intermediary plateau could not be seen when the preincubation time was 10 min and the activation was completed at 10 mM P_i. RuBisCO from Euglena also showed a quite similar activation kinetics. The activation was not due to the contaminating CO_2 included in the stock P_i Solution or in the activation buffer containing the enzyme. The experiments with 2-carboxyarabinitol 1,5-bisphosphate showed that the P_i-stimulated activation was due to the promotion of binding of the activator CO_2 to the activation sites. It was also found that P_i increased the affinity of RuBisCO for the activator CO_2 5.4-fold accompanied by a decrease of the half-saturating concentration of CO_2 to 1.6 μM at 20 mM MgCl_2. Physiological significance of the effects of P_i On the activation of RuBisCO is discussed.
- 日本植物生理学会の論文
著者
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Usuda Hideaki
Laboratory Of Chemistry Teikyo University School Of Medicine
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Usuda Hideaki
Laboratory Of Chemistry Faculty Of Medicine Teikyo University
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Yokota Akiho
Department Of Agricultural Chemistry University Of Osaka Prefecture
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Sawada Shinichi
Department of Biofunctional Science, Faculty of Agriculture and Life Science, Hirosaki University
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Sawada Shinichi
Department Of Biofunctional Science Faculty Of Agriculture And Life Science Hirosaki University
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Sawada Shinichi
Department Of Biology Faculty Of Science Hirosaki University
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Yokota A
Plant Molecular Physiology Research Institute Of Innovative Technology For The Earth (rite)
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Usuda Hideaki
Laboratory Of Chemisry Teikyc University School Of Medicine
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