Induction of the Inactivation and Degradation of Phosphoenolpyruvate Carboxylase and Ribulose-1,5-bisphosphate Carboxylase/Oxygenase in Maize Leaves by Freezing and Thawing : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
スポンサーリンク
概要
- 論文の詳細を見る
When frozen leaves of 24-day-old maize (Zea mays L.) plant were thawed on moist filter paper at 26℃ (freeze-thaw treatment) several enzymes, including phosphoenolpyruvate carboxylase (PEPC) and ribulose-1,5-bisphosphate carboxylase (RuBPC), were rapidly inactivated and degraded. The kinetics of the inactivation and degradation were pseudo first-order, and the half-times for inactivation of PEPC and RuBPC were 3.2 and 2.4 min, respectively. The effect of the freeze-thaw treatment on the inactivation and degradation differed among various enzymes: the residual activities of RuBPC, PEPC, hydroxypyruvate reductase, Cyt c oxidase, NADP-malic enzyme and α-mannosidase 10 min after the start of the thawing treatment were 7, 16, 54, 64, 97 and 98% of the initial respective levels. Thirty min after the starting of thawing treatment, the amounts of total soluble protein, the large subunit of RuBPC, the small subunit of RuBPC, the PEPC subunit and the NADP-malic enzyme subunit had fallen to 61 , 2, 16, 8, and 66% of the initial respective amounts. The effect of freeze-thaw treatment on PEPC was greater in old leaves than in young leaves. There was a steady increase of the rate of degradation of PEPC by freeze-thaw treatment as plants aged from 6 to 24 days. These results are discussed in the context of protein degradation in plant cells.
- 日本植物生理学会の論文
著者
-
Usuda Hideaki
Laboratory Of Chemistry Faculty Of Medicine Teikyo University
-
Shimogawara Kousuke
Laboratory of Chemistry, Teikyo University School of Medicine
-
Shimogawara Kousuke
Laboratory Of Chemistry Faculty Of Medicine Teikyo University
-
Usuda Hideaki
Laboratory Of Chemisry Teikyc University School Of Medicine
関連論文
- Photosynthesis with Single-Rooted Amaranthus Leaves.I.Changes in the Activities of RuBp-1, 5-bisphosphate Carboxylase and Phosphoenolpyruvate Carboxylase and the Amounts of Intermediates in Photosynthetic Metabolism in Response to Changes in the Source-Si
- Binding of Ribulose 1,5-Bisphosphate to the Non-Catalytic Sites of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase and Its Metabolic Implications : PROTEINS, ENZYMES AND METABOLISM
- High Intracellular Phosphorus Contents Exhibit a Correlation with Arsenate Resistance in Chlamydomonas Mutants
- Insertional Mutagenesis in a Homologue of a Pi Transporter Gene Confers Aresenate Resistance on Chlamydomonas
- Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of Chlamydomonas reinhardtii
- Regulation of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Activation by Inorganic Phosphate through Stimulating the Binding of the Activator CO_2 to the Activation Sites : PROTEINS, ENZYMES AND METABOLISM
- Diurnal changes in malate-decarboxylation activity in the leaves of a CAM plant, Bryophyllum daigremontianum
- The Activation State of Ribulose 1,5-bisphosphate Carboxylase in Maize Leaves in Dark and Light
- Changes in the Rate of Photosynthesis during Grain Filling and the Enzymatic Activities Associated with the Photosynthetic Carbon Metabolism in Rice Panicles
- Uptake of Inorganic Phosphate by Suspension-Cultured Tobacco Cells : Kinetics and Regulation by P_i Starvation : ENVIRONMENTAL AND STRESS RESPONSES
- Photosynthesis with Single-Rooted Amaranthus Leaves. II. Regulation of Ribuelose-1,5-Bisphosphate Carboxylase, Phosphoenolpyruvate Carboxylase, NAD-Malic Enzyme and NAD-Malate Dehydrogenase and Coordination between PCR and C4 Photosynthetic Metabolism in
- Phosphate Deficiency in Maize. I. Leaf Phosphate Status, Growth, Photosynthesis and Carbon Partitioning
- Phosphate Deficiency in Maize VI. Changes in the Two-Dimensional Electrophoritic Patterns of Soluble Proteins from Second Leaf Blades Associated with Induced Senescence : GROWTH AND DEVELOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Development of Sink Capacity of the "Storage Root" in a Radish Variety with a Low Ratio of "Storage Root" to Shoot
- Development of Sink Capacity of the "Storage Root" in a Radish Cultivar with a High Ratio of "Storage Root" to Shoot
- The Effects of Increased Atmospheric Carbon Dioxide on Growth, Carbohydrates, and Photosynthesis in Radish, Raphanus sativus
- Phosphate Deficiency in Maize. V. Mobilization of Nitrogen and Phosphorus within Shoots of Young Plants and its Relationship to Senescence : GROWTH AND DEVELOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Participation of Inorganic Orthophosphate in Regulation of the Ribulose-1,5-bisphosphate Carboxylase Activity in Response to Changes in the Photosynthetic Source-Sink Balance : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Regulation of Ribulose-1,5-bisphosphate Carboxylase Activity in Response to Changes in the Source/Sink Balance in Single-Rooted Soybean Leaves: The Role of Inorganic Orthophosphate in Activation of the Enzyme
- Non-Autocatalytic Build-Up of Ribulose 1,5-bisphosphate during the Initial Phase of Photosynthetic Induction in Maize Leaves
- Variations in the Photosynthesis Rate and Activity of Photosynthetic Enzymes in Maize Leaf Tissue of Different Ages
- Induction of the Inactivation and Degradation of Phosphoenolpyruvate Carboxylase and Ribulose-1,5-bisphosphate Carboxylase/Oxygenase in Maize Leaves by Freezing and Thawing : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Phosphate Deficiency in Maize. IV. Changes in Amounts of Sucrose Phosphate Synthase during the Course of Phosphate Deprivation
- Phosphate Deficiency in Maize. II. Enzyme Activities
- Spatial and Temporal Distribution of Sucrose Synthase in the Radish Hypocotyl in Relation to Thickening Growth
- A case study of measles vaccination for university students during the measles outbreak in Tokyo, Japan, 2007
- Search for Photorespiration Inhibitors ; Glycine and Serine Derivatives