Participation of Inorganic Orthophosphate in Regulation of the Ribulose-1,5-bisphosphate Carboxylase Activity in Response to Changes in the Photosynthetic Source-Sink Balance : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
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概要
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Sink-limited conditions, defined as treatment with continuous illumination, cause a reduction in the rate of photosynthetic fixation of CO_2 in single-rooted leaves of soybean (Glycine max. Merr. cv. Turunoko). We suggested previously that this reduction is due to a deactivation of ribulose-1,5-bisphosphate carboxylase (RuBPcase, E.C. 4.1.1.39) that is caused by a decrease in the level of P_i in the leaves [Sawada et al. (1989) Plant Cell Physiol. 30: 691 , Sawada et al. (1990) Plant Cell Physiol. 31 : 697] . In the present study, the mechanism of regulation of RuBPcase activity by Pi was examined. The activity of RuBPcase in the sink-limited leaves, exposed for 6 or 7 d to continuous illumination to alter the source/sink balance, was enhanced with increasing concentrations of Pi in a CO_2-free preincubation medium in the presence of 5 mM MgCl_2. The maximum value [6.3 μmole CO_2 (mg Chl)^<-1> min^<-1>] was obtained at approximately 5 mM P_i after a 5 min incubation, being 3 times of the activity without the preincubation. The activity of a crude preparation of RuBPcase that had been deactivated by removal of CO_2 and Mg^<2+> ions by the gel filtration was 5.2-9.3 nmole CO_2 (mg protein)^<-1> min^<-1> and was also enhanced by P_i Plus Mg^+ ions. The maximum value [147-151 nmole CO_2 (mg protein)^<-1> min<-1>] was attained at 5 mM P_i after a 5 min incubation. The cycle of activation and inactivation of deactivated crude RuBPcase was perfectly reversible by addition of P_i to the enzyme and removal of P_i from the enzyme. Levels of free P_i and of esterified phosphate in the sink-limited leaves were 69% and 31% of the total phosphate, respectively. By contrast, in the control leaves, these values were 87% and 13%, respectively. These results support our previously stated hypothesis and indicate an important role for free P_i in the regulation of RuBPcase activity, in particular in sink-limited plants.
- 日本植物生理学会の論文
著者
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Usuda H
Teikyo Univ. School Of Medicine Tokyo Jpn
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Usuda Hideaki
Laboratory Of Chemistry Faculty Of Medicine Teikyo University
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Sawada Shinichi
Department Of Biofunctional Science Faculty Of Agriculture And Life Science Hirosaki University
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Sawada S
Hirosaki Univ. Hirosaki Jpn
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Tsukui Takahiro
Department of Biology, Faculty of Science, Hirosaki University
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Usuda Hideaki
Laboratory Of Chemisry Teikyc University School Of Medicine
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Tsukui T
Hirosaki Univ. Hirosaki Jpn
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Tsukui Takahiro
Department Of Biology Faculty Of Science Hirosaki University
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