Regulation of Ribulose-1,5-bisphosphate Carboxylase Activity in Response to Changes in the Source/Sink Balance in Single-Rooted Soybean Leaves: The Role of Inorganic Orthophosphate in Activation of the Enzyme
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概要
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The results of our previous study [Sawada et al. (1989) Plant Cell Physiol. 30: 691] implied that, under sink-limited conditions, a decrease in the activity of ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) caused a reduction in the rate of photosynthetic fixation of CO_2 in single-rooted leaves of soybean (Glycine max L. Merr. cv. Tsurunoko). This reduction in the rate of photosynthesis in source leaves seemed to correspond to a decrease in the demand by sink tissues for photoassimilates. In the present study, the activity of RuBPcase in vivo was estimated by measuring the "initial" activity immediately after extraction from standard leaves (grown under a regime of 10 h of light and 14 h of darkness) and from sink-limited leaves (exposed for 6 or 7 d to continuous light to alter the source/sink balance). The rate of photosynthesis in the sink-limited leaves decreased to 47% of that in the standard leaves. The "initial" activity of RuBPcase was 4.3 in the standard leaves but only 1.6 μmole CO_2・(mg Chl)^<-1>・min^<-1> in the sink-limited leaves. These results appear to indicate that the reduction in photosynthetic activity under sink-limited conditions was mostly due to a deactivation of RuBPcase. The activity of deactivated RuBPcase in the sink-limited leaves was restored to 4.1 μmole CO_2・(mg Chl)^<-1>・min^<-1> by incubation of the enzyme in a medium that contained only Na_2HPO_4. This result suggests that free P_i in chloroplasts plays an important role in the activation of the enzyme. The level of P_i in the sink-limited leaves was 62% of that in the standard leaves. On the basis of these various results, it appears that the deactivation of RuBPcase in the sink-limited leaves is the result of a decrease in the level of P_i. The role of free P_i in the activation of RuBPcase, in particular at atmospheric concentrations of CO_2, was also investigated.
- 日本植物生理学会の論文
著者
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Usuda H
Teikyo Univ. School Of Medicine Tokyo Jpn
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Usuda Hideaki
Laboratory Of Chemistry Faculty Of Medicine Teikyo University
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Sawada Shinichi
Department Of Biofunctional Science Faculty Of Agriculture And Life Science Hirosaki University
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Sawada S
Hirosaki Univ. Hirosaki Jpn
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Tsukui Takahiro
Department of Biology, Faculty of Science, Hirosaki University
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Hasegawa Yasuhide
Department of Biology, Faculty of Science, Hirosaki University
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Usuda Hideaki
Laboratory Of Chemisry Teikyc University School Of Medicine
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Tsukui T
Hirosaki Univ. Hirosaki Jpn
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Tsukui Takahiro
Department Of Biology Faculty Of Science Hirosaki University
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Hasegawa Yasuhide
Department Of Biology Faculty Of Science Hirosaki University
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