Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of Chlamydomonas reinhardtii

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概要

• 論文の詳細を見る

• Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.

• 日本植物生理学会の論文

著者

• Usuda H

  Teikyo Univ. School Of Medicine Tokyo Jpn

• Usuda Hideaki

  Laboratory Of Chemistry Teikyo University School Of Medicine

• Usuda Hideaki

  Laboratory Of Chemistry Faculty Of Medicine Teikyo University

• HOSHINO Satoshi

  National Institute of Advanced Industrial Science and Technology

• FUJIWARA Shoko

  School of Life Science, Tokyo University of Pharmacy and Life Science

• TSUZUKI Mikio

  School of Life Science, Tokyo University of Pharmacy and Life Science

• KAISE Toshikazu

  School of Life Science, Tokyo University of Pharmacy and Life Science

• Shimogawara Kosuke

  Laboratory Of Chemistry Teikyo University School Of Medicine

• Kobayashi I

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Tsuzuki Mikio

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Fujiwara Shoko

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Fujiwara S

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Fujiwara Shoko

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Fujiwara Shoko

  Second Department Of Internal Medicine Osaka Medical College

• Fujiwara Shoko

  Department Of Bacteriology Faculty Of Medicine Tottori University

• KOBAYASHI Isao

  School of Life Science, Tokyo University of Pharmacy and Life Science

• Hoshino Satoshi

  School of Life Science, Tokyo University of Pharmacy and Life Science

• Kaise Toshikazu

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Kaise Toshikazu

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Shimogawara K

  Teikyo Univ. School Of Medicine Tokyo Jpn

• Hoshino S

  National Institute Of Advanced Industrial Science And Technology

• Usuda Hideaki

  Laboratory Of Chemisry Teikyc University School Of Medicine

• Tsuzuki Mikio

  School of Life Sciences, Tokyo University of Pharmacy and Life Sciences

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