Effects of Shifting pH in the Stationary Phase of Growth on the Chemical Composition of Euglena gracilis

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Effects of pH shifted in the stationary phase on the chemical composition of Euglena gracilis cells were examined. When the cells that were cultivated at fixed pH of 4.5 reached stationary phase, the pH of the culture was shifted to 2.0-8.5, and the cultivation was continued for 24 hours more. Shifting pH to below 3.0 and between 6.5 and 7.5 increased cellular protein and decreased paramylon. The maximum content of protein in the cells was 77.5% when the pH was shifted to 7.5. At shifted pH of higher than 8.0, the protein content was greatly decreased and the paramylon content was greatly increased. This technique of shifting pH in the stationary phase did not improve the lipid content or fatty acid composition in Euglena for use as a feed for larval fish. Arachidonic acid, however, was found to be decreased in alkaline pH after shifting. This pH shifting technique that can improve the protein content in Euglena cells is useful for mass production of Euglena for use in the feed industry.
社団法人日本農芸化学会の論文
1994-11-23