Monitoring the Irradiation-induced Conformational Changes of Ovalbumin by Using Monoclonal Antibodies and Surface Plasmon Resonance
スポンサーリンク
概要
- 論文の詳細を見る
Two types of conformationally specific anti-irradiated ovalbumin monoclonal antibodies were prepared in order to study and monitor irradiation-induced structural changes in the ovalbumin molecule. Surface plasmon resonance (SPR) detection was used to investigate the kinetic parameters of the reaction between antibodies and ovalbumin which had been administered with different doses of irradiation (0,1.5,2.0,5.0,10,20,50,and 100 kGy). The results demonstrate that the combination of monoclonal antibodies and the SPR method can be used to characterize the irradiation-induced conformational change with an unlabelled reagent.
- 社団法人日本農芸化学会の論文
- 2000-04-23
著者
-
YASUMOTO Kyoden
Department of Human Nutrition, School of Life Studies, Sugiyama Jogakuen University
-
Kitabatake Naofumi
Research Institute for Food Science, Kyoto University
-
Kitabatake Naofumi
Research Institute For Food Science Kyoto University
-
Masuda Tomomi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
-
Masuda T
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
-
Masuda Takayuki
Department Of Agricultural Chemistry Kinki University
-
Kitabatake Naofumi
Research Institute For Food Science Kyoto University Gokasho
-
Masuda Tetsuya
Research Institute For Mathematical Sciences Kyoto University
-
Yasumoto Kyoden
Department Of Food And Nutrition School Of Life Studies Sugiyama Jogakuen University
-
Yasumoto Kyoden
Department Of Food And Nutrition Sugiyama Jogakuen University
-
Masuda Tetsuya
Research Institute for Food Science, Kyoto University Gokasho
関連論文
- Elevated Urinary Cr Loss Induces a Reduction in Renal Cr Concentration and the Negative Cr Balance in Streptozotocin-Induced Diabetic Mice
- Heat-induced Transparent Gel from Hen Egg Lysozyme by a Two-step Heating Method
- Heat-induced Transparent Gel Formation of Bovine Serum Albumin
- Effects of Tripalmitin and Saffiower Oil on the Content of Short-chain Fatty Acids in the Cecum and on the Fecal Excretion of Lipids in Rats
- Effects of Corn Oil and Pectin on the Production of Short-chain Fatty Acids in the Cecum, on the Growth of Colonic Bacteria, and on the Liver Cholesterol Level in Rats
- Effects of Gum Arabic and Pectin on the Emulsification, the Lipase Reaction, and the Plasma Cholesterol Level in Rats(Food & Nutrition)
- Purification and Characterization of Aromatic Amine Dehydrogenase from Alcaligenes xylosoxidans
- Effects of Pectin and Lard on the Production of Short-chain Fatty Acids in the Cecum, on the Growth of Colonic Bacteria, and on the Liver Cholesterol Level in Rats(Food & Nutrition)
- Role of the Carbohydrate Chain and Two Phosphate Moieties in the Heat-Induced Aggregation of Hen Ovalbumin
- Enzymatic Synthesis of Novel Disaccharides Using Disaccharide Phosphorylases
- Purification and Characterization of Trehalose Phosphorylase from Catellatospora ferruginea
- Properties of Maltose Phosphorylase from Propionibacterium freudenreichii
- Three-Dimensional Structure of Mucosal and Submucosal Lymphatics in Rat Small Intestine
- Screening for Tyrosinase Inhibitors among Extracts of Seashore Plants and Identification of Potent Inhibitors from Garcinia subelliptica
- Cytotoxic Screening of Medicinal and Edible Plants in Okinawa, Japan, and Identification of the Main Toxic Constituent of Rhodea japonica (Omoto)(Organic Chemistry)
- Isolation and Antioxidant Activity of Galloyl Flavonol Glycosides from the Seashore Plant, Pemphis acidula
- Occurrence of the Major Food Allergen, Ovomucoid, in Human Breast Milk as an Immune Complex
- Deglycosylation of Hen Ovalbumin in Native Form by Endo-β-N-Acetylglucosaminidase(Food & Nutrition)
- Physicochemical and Functional Properties of Hen Ovalbumin Dephosphorylated by Acid Phosphatase(Food & Nutrition)
- Promotion of Germination and Shoot Elongation of Some Plants by Alginate Oligomers Prepared with Bacterial Alginate Lyase
- Toxicity of Dimethyl Sulfoxide as a Solvent in Bioassay System with HeLa Cells Evaluated Colorimetrically with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide(Biological Chemistry)
- Turbidity and Rheological Properties of Gels and Sols Prepared by Heating Process Whey Protein
- Improved Swimming Pool Achieves Higher Reproducibility and Sensitivity to Effect of Food Components as Ergogenic Aids
- Flow Cytometric Estimation of Cytotoxic Activity of Rhodexin A Isolated from Rhodea japonica in Human Leukemia K562 Cells
- Monitoring the Irradiation-induced Conformational Changes of Ovalbumin by Using Monoclonal Antibodies and Surface Plasmon Resonance
- Tyrosinase Inhibitory Activity of Ethanol Extracts from Medicinal and Edible Plants Cultivated in Okinawa and Identification of a Water-Soluble Inhibitor from the Leaves of Nandina domestica
- Cytotoxic Activity of Maytanprine Isolated from Maytenus diversifolia in Human Leukemia K562 Cells(Toxicology)
- Purification and Characterization of Serine Hydroxymethyltransferase from Mitochondria of Euglena gracilis z
- Effects of D-Amino Acids on Cytosolic Serine Hydroxymethyltransferase from Euglena gracilis z
- Purification and Characterization of Cytosolic Serine Hydroxymethyltransferase from Euglena gracilis z(Biological Chemistry)
- Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions
- Cytotoxicity of Citrinin Heated at Temperatures above 100℃
- Sweetness of Sweet Protein Thaumatin Is More Thermoresistant under Acid Conditions Than under Neutral or Alkaline Conditions
- Method for the Accurate Measurement of Freezing-induced Denaturation of Ovalbumin with 5,5'-Dithiobis-(2-nitrobenzoic acid)
- Thermal Denaturation of Soybean Protein at Low Water Contents(Food & Nutrition)
- Denaturation Temperature of Soy Protein under Low Moisture Conditions(Food & Nutrition)
- Protective Effects of Non-ionic Surfactants against Denaturation of Rabbit Skeletal Myosin by Freezing and Thawing(Food & Nutrition)
- Amino Acid Composition of Soybean Protein Increased Postprandial Carbohydrate Oxidation in Diabetic Mice(Food & Nutrition Sience)
- Molecular Interaction between Proteins Involved in EvgAS Signal Transduction of Escherichia coli
- Effects of Saliva on the Viscosity of Gum Solutions
- Zinc Bioavailability Is Improved by the Micronised Dispersion of Zinc Oxide with the Addition of L-Histidine in Zinc-Deficient Rats
- Properties of Chlorella cells grown under various photo-heterotrophic conditions
- Turbidity and Hardness of a Heat-induced Gel of Hen Egg Ovalbumin(Food & Nutrition)
- Heat-induced and Transparent Gel Prepared from Hen Egg Ovalbumin in the Presence of Salt by a Two-step Heating Method(Food & Nutrition)
- Distortion of Periodontal Tissue in Maxillary Molar Region during Biting-Measurement with Quasi Three-Dimensional Method-
- Positive Cones and $L_p$-Spaces for von Neumann Algebras
- $L\sb{p}$-Spaces for von Neumann Algebra with Reference to a Faithful Normal Semifinite Weight
- A Note on a Theorem of A. Connes on Radon-Nikodym Cocycles
- Groupoid Dynamical Systems and Crossed Product, I-The Case of $W\sp *$-Systems
- A Major Decomposition Product, Citrinin H2, from Citrinin on Heating with Moisture(Food & Nutrition Science)
- Measurement of Temperature of Food Materials during Extrusion Cooking
- Application of Fourier Hyperfunction to Fourier Expansion in Continuous Crossed Product(Generalized Functions and Linear Differential Equations, VIII)
- Scanning Electron Microscopy of Freeze-dried Protein Foams
- Chaotropic Ions in Activation and Protection of γ-Glutamyltransferase from Fruiting Bodies of Lentinus edodes
- Daily Rhythmic Changes in Amino Acid Transport in Isolated Intestinal Epithelial Cells from Rats on Restricted-feeding Regimen
- Distribution of Selenium in Bovine Milk and Selenium Deficiency in Rats Fed Casein-based Diets, Monitored by Lipid Peroxide Level and Glutathione Peroxidase Activity
- Crcadian Rhythmicity of Glycyl-L-leucine Absorption Mechanism in Rat Small Intestine
- Purification and Immunochemical Analysis of Rat Liver Glutathione Peroxidase
- Glutathione Oxidase from Bovine Skim Milk Membranes: Its Copurification with γ-Glutamyltransferase
- Bioavailability of Selenite, Selenomethionine and Selenocystine in Rats with Silver Loading
- Substrate Preference of γ-Gluta-myltransferase from Caps of Fruiting Bodies of Lentinus edodes
- In Vitro and In Situ Absorption of Methionine Sulfoxide in Rat Small Intestine