Toxicity of Dimethyl Sulfoxide as a Solvent in Bioassay System with HeLa Cells Evaluated Colorimetrically with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide(Biological Chemistry)

概要

論文の詳細を見る
The toxicity of dimethyl sulfoxide (Me_2SO) was examined in HeLa cells cultured at 37℃ for up to 72 hr. The growth of the cells was measured by a colorimetric method with the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyItetrazolium bromide (MTT), which gave good correlation between the cell number and the color development from the reduction of MTT under suitable conditions. When the initial number of cells was 3×10^4/ml, Me_2SO at 1% or less had no apparent effect on prolitiration for up to 48 hr of incubation, but in longer incubations, cell growth was repressed. When the initial number of cells was 3×10^5/ml, the effect of Me_2SO was similar.
社団法人日本農芸化学会の論文
1990-11-23