Lysosomal nature of plant vacuoles I. Apparent absence of lysosomal particles in tomato fruit and leaf homogenates

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Differential centrifugation of the subcellular particles of tomato fruit was carried out after brief homogenization of the tomato fruit with a blendor in 0.5 M sucrose, containing 0.2 M Tris-HCl buffer, pH 8.0. About 60% of the catalase activity was detected in the peroxisomal fraction. However, most of the acid phosphatase and carboxypeptidase, which are marker enzymes for lysosomes, were detected in the soluble fraction, as was glucose-6-phosphate dehydrogenase, a marker enzyme for soluble cytosol. Similar results were obtained from differential centrifugation of the tomato leaf homogenate. This absence of the lysosomal particles in the tomato fruit and leaves is discussed, particularly as an indication of the presence of hydrolytic enzymes in the central vacuoles of mature plant cells.
日本植物生理学会の論文