Separation of Subunit Polypeptides of Glutenin by SDS-PAGE and Determination of Their Cysteine Contents
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概要
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The main subunits of glutenin were separated by preparative SDS-PAGE with a Laemmli system (U. K. Laemmli, Nature, 227, 680 (1970)) and their cysteine (Cys) contents were determined by amino acid analysis. Aminoacid compositions of glutenin subunits, determined in the present study, were different from those determined by Danno et al. [G. Danno, K. Kanazawa and M. Natake, Agric. Biol. Chem., 40, 739 (1976)]. Wefound that these differences were due to the different methods of hydrolysis of subunit polypeptides. That is, hydrolysis of subunit polypeptides extracted from gel and hydrolysis of polypeptides in gel without extraction. Cys contents of glutenin subunits were determined as S-pyridylethyl cysteine (PE-Cys). Although no PE-Cys was detected in B-4 or B-4, all other subunits were shown to have 4mol Cys per mol protein, respectively.
著者
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Matoba Teruyoshi
Research Institute For Food Science Kyoto University
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Matsumura Yasuki
Research Institute For Food Science Kyoto University
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KAWAMURA Yukio
Research Institute for Food Science, Kyoto University
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YONEZAWA Daizo
Faculty of Domestic Science, Mukogawa Womens University
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