Cloning of the Gene Cluster Responsible for Biosynthesis of KS-505a (Longestin), a Unique Tetraterpenoid

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概要

• 論文の詳細を見る

• KS-505a (longestin), produced by Streptomyces argenteolus, has a unique structure that consists of a tetraterpene (C40) skeleton, to which a 2-O-methylglucuronic acid and an o-succinyl benzoate moiety are attached. It is a novel inhibitor of calmodulin-dependent cyclic-nucleotide phosphodiesterase, which is representative of a potent anti-amnesia drug. As a first step to understanding the biosynthetic machinery of this unique and pharmaceutically useful compound, we cloned a KS505a biosynthetic gene cluster. First we searched for a gene encoding octaprenyl diphosphates, which yielded a C40 precursor by PCR, and four candidate genes were obtained. Among these, one was confirmed to have the expected enzyme activity by recombinant enzyme assay. On the basis of an analysis of the flanking regions of the gene, a putative KS-505a biosynthetic gene cluster consisting of 24 ORFs was judged perhaps to be present on a 28-kb DNA fragment. A gene disruption experiment was also employed to confirm that the cluster indeed participated in KS-505a biosynthesis. This is believed to be the first report detailing the gene cluster of a cyclized tetraterpenoid.

• 社団法人 日本農芸化学会の論文
• 2007-12-23

著者

• SETO Haruo

  Faculty of Applied Biosciences, Tokyo University of Agriculture

• Itoh N

  Biotechnology Research Center Toyama Prefectural University

• Itoh Nobuya

  Biotechnology Research Center Toyama Prefectural University

• DAIRI Tohru

  Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University

• ONAKA Hiroyasu

  Biotechnology Research Center, Toyama Prefectural University

• HAYASHI Yutaka

  Biotechnology Research Center, Toyama Prefectural University

• Hayakawa Yoichi

  Institute Of Low Temperature Science Hokkaidou University

• Yamashita H

  Biotechnology Research Center Toyama Prefectural University

• Hayakawa Y

  Institute Of Low Temperature Science Hokkaido University

• Hayakawa Y

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Seto H

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Onaka Hiroyasu

  Dep. Of Biotechnology Fac. Of Engineering And Biotechnology Res. Center Toyama Prefectural Univ.

• Onaka Hiroyasu

  Biotechnology Research Center Toyama Prefectural University

• Dairi T

  Biotechnology Res. Center Toyama Prefectural Univ.

• Dairi Tohru

  Biotechnology Research Center Toyama Prefectrual University

• Seto H

  Faculty Of Applied Bioscience Tokyo University Of Agriculture

• Seto Haruo

  Department Of Applied Biology And Chemistry Tokyo University Of Agriculture

• Dairi Tohru

  Hokkaido Univ. Hokkaido Jpn

• Hayakawa Yoichi

  Institute Of Applied Microbiology The University Of Tokyo

• Hayashi Yutaka

  Biotechnology Research Center Toyama Prefectural University

• Hayakawa Yoichi

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Seto Haruo

  Faculty of Applied Bio-science, Tokyo University of Agriculture

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