Bacillus subtilis ypgA Gene Is fni, a Nonessential Gene Encoding Type 2 Isopentenyl Diphosphate Isomerase
スポンサーリンク
概要
- 論文の詳細を見る
We previously identified the fni gene of Streptomyces sp. strain CL190 as type 2 isopentenyl diphosphate (IPP) isomerase, which needs both FMN and NADPH for enzyme activity. An fni gene homolog, ypgA, was detected in the database of the Bacillus subtilis genome. However, the ypgA product was about 140 amino acids shorter in the N-terminal than the Streptomyces fni gene product. A database search found three new putative start codons in 129, 225, and 411 bases upstream of the original start codon of the ypgA gene. The longest gene product, which was named ypgA3, showed the most significant homology to the Streptomyces fni gene product. The ypgA3 gene was expressed with an N-terminal His-tag in Escherichia coli and the purified soluble protein was characterized in detail. The ypgA3 protein converted IPP to its isomer dimethylallyl diphosphate in the presence of both FMN and NADPH. The enzyme also catalyzed the reverse reaction in the presence of both the cofactors. Disruption of the ypgA3 gene was not lethal to B. subtilis. These results indicate that Bacillus ypgA3 gene is fni, a nonessential gene encoding type 2 IPP isomerase.
- 2004-01-23
著者
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HAYAKAWA YOICHI
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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SETO Haruo
Institute of Molecular and Cellular Biosciences, The University of Tokyo
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Takagi Motoki
Biomedicinal Information Research Center (birc) Japan Biological Informatics Consortium (jbic)
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Takagi Motoki
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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KANEDA Kazuhide
Institute of Molecular and Cellular Biosciences, University of Tokyo
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SHIMIZU Tomohiro
Institute of Molecular and Cellular Biosciences, University of Tokyo
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KUZUYAMA Tomohisa
Institute of Molecular and Cellular Biosciences, University of Tokyo
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Hayakawa Yoichi
Institute Of Low Temperature Science Hokkaidou University
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Hayakawa Yoichi
Institute Of Low Temperature Science Hokkaido University
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Kawasaki Takashi
Biotechnology Research Center Toyama Prefectural University
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Yamashita H
Biotechnology Research Center Toyama Prefectural University
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Hayakawa Y
Institute Of Low Temperature Science Hokkaido University
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Hayakawa Y
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Seto H
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Hamano Yoshimitsu
Biotechnology Research Center Toyama Prefectural University
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Kunigami T
Biotechnology Research Center Toyama Prefectural University
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Hayakawa Y
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
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Kaneda Kazuhide
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Seto Haruo
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Seto H
Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Seto Haruo
Department Of Applied Biology And Chemistry Tokyo University Of Agriculture
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Kuzuyama T
Univ. Tokyo Tokyo
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Kuzuyama Tomohisa
Biotech. Research Center Tokyo Univ.
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Kuzuyama Tomohisa
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Hayakawa Yoichi
Institute Of Applied Microbiology The University Of Tokyo
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Seto Haruo
Institute Of Applied Microbiology The University Of Tokyo
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Shimizu Tomohiro
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Hayakawa Yoichi
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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