Cloning and Expression in Escherichia coli of 2-Hydroxypropylphosphonic Acid Epoxidase from the Fosfomycin-producing Organism, Pseudomonas syringae PB-5123

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概要

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• The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase from fosfomycin-producing Streptomyces wedmorensis. The purified recombinant protein of this ORF converted 2-hydroxypropylphosphonic acid to fosfomycin. This result clearly showed the ORF to encode 2-hydroxypropylphosphonic acid epoxidase in PB-5123.

• 社団法人日本農芸化学会の論文
• 1999-12-23

著者

• SETO Haruo

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• Seki T

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• KUZUYAMA Tomohisa

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• Seki Taiichiro

  Lab. Of Nutrition And Physiology Dep. Of Chemistry And Life Sci. Nihon Univ. Coll. Of Bioresource Sc

• Kobayashi S

  Kyoto Univ. Kyoto Jpn

• SEKI Takayuki

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• KOBAYASHI Seiji

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• HIDAKA Tomomi

  Center for Gene Research, Ehime University

• Kawasaki Takashi

  Biotechnology Research Center Toyama Prefectural University

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• Hidaka T

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Kunigami T

  Biotechnology Research Center Toyama Prefectural University

• Seto Haruo

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Seto H

  Faculty Of Applied Bioscience Tokyo University Of Agriculture

• Kuzuyama T

  Univ. Tokyo Tokyo

• Kuzuyama Tomohisa

  Biotech. Research Center Tokyo Univ.

• Kuzuyama Tomohisa

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Seto Haruo

  Institute Of Applied Microbiology The University Of Tokyo

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