Thermostability of Allicin Determined by Chemical and Biological Assays
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概要
- 論文の詳細を見る
The garlic-derived antibacterial principle, alk(en)yl sulfinate compounds, has long been considered as very short-lived substance. However, there are some data showing a rather more stable nature of allicin. We determined here the thermostability of allicin by a systematic analyses employing chemical quantification and an antibacterial activity assay. Allicin in an aqueous extract of garlic was degraded stoichiometrically in proportion to the temperature; we estimated the half-life of allicin to be about a year at 4 °C (from 1.8 mg/ml to 0.9 mg/ml) and 32 d at 15 °C, but only 1 d at 37 °C (from 2.0 mg/ml to 1.0 mg/ml). The half-life values for antibacterial activity showed a similar trend in results: 63 d or more at 4 °C for both antibacterial activities, 14 d for anti-staphylococcal activity, and 26 d for anti-escherichia activity at 15 °C, but only 1.2 d and 1.9 d for the respective activities at 37 °C. Such antibacterial activities were attributable to the major allicin, allyl 2-propenylthiosulfinate. Surprisingly, the decline in the quantity of allicin was not accompanied by its degradation; instead, allicin became a larger molecule, ajoene, which was 3-times larger than allicin.
- 社団法人 日本農芸化学会の論文
- 2008-11-23
著者
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Seki T
Institute Of Molecular And Cellular Biosciences University Of Tokyo
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Seki Taiichiro
Lab. Of Nutrition And Physiology Dep. Of Chemistry And Life Sci. Nihon Univ. Coll. Of Bioresource Sc
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Seki T
Department Of Nutrition And Physiology Nihon University College Of Bioresource Sciences
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Seki T
Nihon Univ. Coll. Of Bioresource Sci. Fujisawa Jpn
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Seki Taiichiro
Laboratory Of Nutrition And Physiology Department Of Agricultural And Biological Chemistry Nihon Uni
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Ariga Toyohiko
Nihon Univ. Graduate School Of Applied Life Sciences
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Ariga Toyohiko
Department Of Agricultural And Biological Chemistry Nihon University College Of Bioresouce Sciences
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関 敏美
Research Division Teikoku Hormone Manufacturing Co. Ltd.
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FUJISAWA Hiroyuki
Nihon University Graduate School of Applied Life Sciences
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SUMA Kaoru
Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
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ORIGUCHI Kana
Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
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SEKI Taiichiro
Nihon University Graduate School of Applied Life Sciences
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Suma Kaoru
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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Origuchi Kana
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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