Microbial Conversion of DL-5-Substituted Hydantoins to the Corresponding L-Amino Acids by Pseudomonas sp. Strain NS671
スポンサーリンク
概要
- 論文の詳細を見る
A bacterial strain, NS671, which converts DL-5-(2-methylthioethyl)hydantoin stereospecifically to L-methionine, was isolated from soil and was classified into the genus Pseudomonas. With growing cells of Pseudomonas sp. strain NS671, DL-5-(2-methylthioethyl)hydantoin was effectively converted to L-methionine. Under adequate conditions, 34 g of L-methionine per liter was produced with a molar yield of 93% from _<DL>-5-(2-methylthioethyl)hydantoin added successively. In addition to L-methionine, other amino acids such as L-valine, L-leucine, L-isoleucine, and L-phenylalanine were also produced from the corresponding 5-substituted hydantoins, but these L-amino acids produced were partially consumed by strain NS671. The hydantoinase, by which 5-substituted hydantoin rings are opened, was ATP-dependent. The N-carbamyl-amino acid amidohydrolase was found to be strictly L-specific, and its activity was inhibited by high concentration of ATP.
- 社団法人日本農芸化学会の論文
- 1993-06-23
著者
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Nakamura H
Sci. Univ. Tokyo Tokyo Jpn
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NAKAMURA Hidemitsu
Department of Biotechnology, The University of Tokyo
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Ishikawa Tomohiro
Department Of Agricultural Chemistry School Of Agriculture Nagoya University
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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Kobayashi S
Kyoto Univ. Kyoto Jpn
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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ISHIKAWA Takahiro
Odawara Research Center, Nippon Soda Co., Ltd.
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WATABE Ken
Odawara Research Center, Nippon Soda Co., Ltd.
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MUKOHARA Yukuo
Odawara Research Center, Nippon Soda Co., Ltd.
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NAKAMURA Hiroaki
Odawara Research Center, Nippon Soda Co., Ltd.
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Nakamura H
Department Of Biotechnology The University Of Tokyo
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Kobayashi Shinobu
Odawara Research Center, Nippon Soda Co., Ltd.
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Mukohara Yukuo
Odawara Research Center Nippon Soda Co. Ltd.
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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