Purification, Characterization, and Sequencing of Novel Antimicrobial Peptides, Tu-AMP 1 and Tu-AMP 2, from Bulbs of Tulip (Tulipa gesneriana L.)
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概要
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Novel antimicrobial peptides (AMP), designated Tu-AMP 1 and Tu-AMP 2, were purified from the bulbs of tulip (Tulipa gesneriana L.) by chitin affinity chromatography and reverse-phase high-performance liquid chromatography (HPLC). They bind to chitin in a reversible way. They were basic peptides having isoelectric points of over 12. Tu-AMP 1 and Tu-AMP 2 had molecular masses of 4,988 Da and 5,006 Da on MALDI-TOF MS analysis, and their extinction coefficients of 1% aqueous solutions at 280 nm were 3.3 and 3.4, respectively. Half of all amino acid residues of Tu-AMP 1 and Tu-AMP 2 were occupied by cysteine, arginine, lysine, and proline. The concentrations of peptides required for 50% inhibition (IC50) of the growth of plant pathogenic bacteria and fungi were 2 to 20 μg/ml. The structural characteristics of Tu-AMP 1 and Tu-AMP 2 indicated that they were novel thionin-like antimicrobial peptides, though Tu-AMP 2 was a heterodimer composes of two short peptides joined with disulfide bonds.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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Minami Y
Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University
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Fujimura M
Fac. Of Life Sciences Toyo Univ.
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Minami Yuji
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Watanabe K
Tokyo Institute Of Technology Faculty Of Bioscience And Biotechnology Department Of Biological Scien
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TADERA Kenjiro
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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Minami Y
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Minami Yuji
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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FUJIMURA Masatoshi
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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IDEGUCHI Mineo
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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Takezaki K
Department Of Biochemical Science And Technology
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Ideguchi Mineo
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Tadera K
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Tadera Kenjiro
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Watanabe K
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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Fujimura M
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Watanabe Keiichi
The Graduate School Of Science And Technology Kobe University
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Tadera Kenjiro
Department of Biochemical Science and Technology
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TADERA Kenjiro
Department of Agricultural Chemistry, Faculty of Agriculture, Kagoshima University
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