IMMUNOCYTOCHEMICAL AND ENZYME-CYTOCHEMICAL LOCALIZATION OF INTESTINAL ALKALINE PHOSPHATASE OF RATS
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概要
- 論文の詳細を見る
Two different techniques, enzyme-cytochemical and immunocytochemical, have been employed to localize alkaline phosphatase in the rat small intestine at light and electron microscopic levels. In the absorptive cells of the duodenum and jejunum, alkaline phosphatase has been localized by the classical enzyme-cytochemical technique on the surfaces of apical microvilli and lateral membranes; on the other hand the direct immunoperoxidase method revealed some additional sites of the enzyme such as cisternae of rough endoplasmic reticulum and perinuclear spaces. Both enzyme- and immunocytochemistry failed to demonstrate the enzyme in the absorptive cells of the ileum. These immunocytochemical findings suggest that the absorptive cells in the proximal small intestine synthesize and tramsport the enzyme protein to their cell surfaces.
- 日本組織細胞化学会の論文
著者
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Nagura H
Department Of Pathology Sendai City Hospital
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NAGURA Hiroshi
Department of Pathology, Tohoku University Hospital
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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YOSHIMURA Shinichi
Cell Biol Res. Lab., Tokai Univ., Sch. of Med.
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Yoshimura Shinichi
Cell Biol. Res. Lab. Tokai Univ. Sch. Med.
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Hasegawa H
Laboratory Of Structure And Function Research Tokai University School Of Medicine
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HASEGAWA Hideaki
Cell Biol. Res. Lab.
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AIHARA KOHJI
Cell Biology Research Laboratoty, Tokai University School of Medicine
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Aihara Kohji
Cell Biology Research Laboratory And Department Of Pathology Tokai University School Of Medicine
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Nagura Hiroshi
Department Of Neurology Tokyo Metropolitan Geriatric Hospital
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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