ENZYME- AND IMMUNO-HISTOCHEMICAL LOCALIZATION OF HUMAN PLACENTAL ALKALINE PHOSPHATASE
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概要
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The localization of human placental alkaline phosphatase (HPALP) was demonstrated by both conventional enzyme-histochemistry and immuno-histochemistry of the indirect peroxidase-labeled antibody method at light and electron microscopic levels. The location of the HPALP was exclusively confined to the microvilli of syncytiotrophoblastic cells by both methods. In early gestational ages, the distribution of the enzyme reaction positive villi was not uniform. At full term, all the syncytiotrophoblastic cells of chorionic villi uniformly showed the most intense staining for the alkaline phosphatase.On tissue sections of the chorionic villi, the HPALP was distinguished from the isoenzymes of small intestine and kidney by the bio-, physico- and immunochemical properties, e.g.: the sensitivity to the stereospecific inhibitor, the L-phenylalanine (0.05M), heat stability (65℃), and the specific reactivity to the anti-HPALP. The specificity and validity of the immunohistochemical staining at the surfaces of the chorionic villi, especially at an ultrastructurallevel, are throughly discussed. It is suggestive of the usefulness of this immunohistochemical staining method for the investigation of Regan type isoenzyme produced in certain malignant tumors.
- 日本組織細胞化学会の論文
著者
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KAMEYA Toru
Department of Pathology, Shizuoka Cancer Center
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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Kameya Toru
Department Of Pathology School Of Medicine Keio University
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Kameya Toru
Department Of Neurosurgery Tokyo Dental College
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KOBAYASHI TAKAYOSHI
Research Laboratory No. 1 Hoechst Japan
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MUKOJOIMA TATSU
Division of Clinical Laboratory, National Cancer CenterHospital
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Mukojima Tatsu
Division Of Clinical Laboratory National Cancer Centerhospital
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Watanabe Keiichi
Department Of Pathology School Of Medicine Keio University
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Kameya Toru
Department Of Cardio-angiology Kitasato University School Of Medicine
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WATANABE Keiichi
Department of Pathology , School of Medicine, Keio University
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