α-Calcitonin Gene-Related Peptide (α-CGRP) and Alternative RNA Processing Events in Calcitonin/α-CGRP Gene Expression of Human and Rat Lung
スポンサーリンク
概要
- 論文の詳細を見る
Calcitonin (CT) and α-calcitonin gene-related peptide (α-CGRP) are generated by alternative RNA processing from a single CT/α-CGRP gene. The expression of CT/α-CGRP gene in small cell and large cell human lung carcinoma cell lines was recently reported and considered to be ectopic production in the lung. In the present study, we first demonstrated the existence of CGRP immunoreactivity in endocrine cells of non-neoplastic lung by immunohistochemical tests with antisera against synthetic α-CGRP. CGRP positive cells were found both as single cells and in groups, which are called neuroepithelial bodies, in human and rat fetal to adult lungs. The peptide was often coexistent with CT and/or gastrin releasing peptide (GRP) in the same cells of the human lungs. In contrast, CT and GRP immunoreactivity was not detected in the rat lung. In order to confirm the presence of CT and α-CGRP production in rat fetal lungs, we examined for the presence of CT and α-CGRP mRNA by the Northern hybridization technique using synthetic probes specific for CT and α-CGRP mRNA. With the α-CGRP probe, we detected the 1.0-kilobase (kb) poly (A)^+ RNA corresponding to mature α-CGRP mRNA together with 1.6- and 3.4-kb poly (A)^+ RNA. The CT probe hybridized with 1.6- and 3.4-kb poly(A)^+ RNA but 0.8-0.9-kb mature CT mRNA was not detected. We identified for the first time the mature 1.0-kb α-CGRP mRNA and the alternative RNA processing events in rat CT/α-CGRP gene expression of rat fetal lungs.
- 北里大学の論文
- 1987-10-31
著者
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KAMEYA Toru
Department of Pathology, Shizuoka Cancer Center
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Kameya Toru
Department Of Pathology Kitasato University School Of Medicine
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Yamaguchi Ken
National Cancer Center Research Institute
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Miki Keizaburo
Tonen Fundamental Research Laboratory
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ONO Masao
Department of Public Health, Aichi Prefecture
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Ono Masao
Department Of Experimental Immunology And Crest Program Of Jst Institute Of Development Aging And Ca
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Kameya T
Kitasato Univ. School Of Medicine
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Kameya Toru
Department Of Neurosurgery Tokyo Dental College
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Wada Chieki
Department of Pathology Kitasato University School of Medicine
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Wada C
Kitasato Univ. School Of Medicine
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Wada Chieki
Department Of Clinical Pathology Kitasato University School Of Medicine
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Ono M
Kitasato Univ. Kanagawa Jpn
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Ono Masao
Department Of Biotechnology Faculty Of Engineering Okayama University
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Kameya Toru
Department Of Cardio-angiology Kitasato University School Of Medicine
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