Alterations in Glutathione Peroxidase Activity following Reperfusion Injury to Rat Liver
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概要
- 論文の詳細を見る
Prevention of cellular damage after warm ischemia is of major importance in liver transplantation. In this study, we determined the extent to which lipid peroxides contribute to the pathogenesis of hepatic cell damage induced by transient warm ischemia with subsequent reperfusion. In addition, the function and immunohistochemical features of glutathione peroxidase, a potent physiological lipid peroxide scavenger of the liver, was assessed. Reperfusion following 15 or 30 minutes of warm ischemia resulted in a significant elevation in serum and liver lipid peroxidase (LPO) levels. In addition, necrosis of the hepatic periportal area accompanied with remarkable rises in serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed. In contrast, 30 min of ischemia without reperfusion caused minimal hepatocellular damage. The adverse changes after ischemia/reperfusion were minimized by pretreatment with superoxide dismutase (SOD). These results indicate that increased lipid peroxidation by production of radicals after reperfusion caused the liver cell damage. After ischemia/reperfusion, liver gulthathione peroxidase (GSH-PO) activity was significantly decreased and its location altered in the damaged liver. These findings suggest that GSH-PO contributes significantly to the protection against hepatic reperfusion injuries.
- 東海大学の論文
著者
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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Yasuda Seiei
東海大学 医学部外科学
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Yasuda Seiei
Department Of Surgery Tokai University School Of Medicine
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Yokoyama Seishichi
Department Of Surgery Tokai University School Of Medicine
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Mitomi Toshio
Department Of Surgery Tokai University School Of Medicine
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Mitomi Toshio
Department Of 2nd Surgery School Of Medicine Tokai University
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Yokoyama Seishichi
Department Of General Surgery School Of Medicine Tokai University
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KATAYAMA Tokitaka
Department of Surgery Tokai University School of Medicine
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Watanabe Keiichi
Department Of Pathology Tokai University School Of Medicine
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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