Immunolocalization of Glutathione-peroxidase and Androgen Receptors in the Rat Dorsolateral Prostate
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概要
- 論文の詳細を見る
The effects of testosterone (T) and 17β-estradiol (E_2) on the dorsolateral prostate of castrated rats were investigated by histopathological and immunohistochemical procedures. A significant increase in the prostatic weight of the castrated rats occurred after treatment with testosterone alone, or in combination with E_2. Histologically, glandular hypertrophy was observed and fibromuscular stromal proliferation was also seen in the rats receiving the combination treatment. Immunohistochemical localization of glutathione-peroxidase (GSH-PO), an enzyme which effectively reduces the lipid peroxides, was clearly detected in the glandular epithelial cells of the dorsolateral prostate following testosterone alone or testosterone plus E_2-treatment Therefore, it appeared that GSH-PO protein synthesis in the glandular epithelium of the dorsolateral prostate can be enhanced (induced?) by sex hormones such as testosterone and E_2. In the dorsolateral prostate of intact rats, positive staining for androgen receptors (AR) was observed in nuclei of the glandular epithelial cells. In addition, immunodetectable AR decreased within 2 days after castration, but returned to intact levels after administration of testosterone. These findings agree with previous work on the prostate. It is concluded that immunohistochemical analysis is very useful in studying the effects of androgen treatment on the prostate.
- 東海大学の論文
著者
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Watanabe K
Aoba Gakuen
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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MIZOKAMI Atsushi
Department of Urology
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Suzuki Minoru
Safety Resarch Department, Teikoku Hormone Mfg Co., Ltd.
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Mikami T
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Tagawa Masashi
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Mizokami Atsushi
Department Of Integrative Cancer Therapy And Urology Kanazawa University Graduate School Of Medical
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Mizokami Atsushi
Department Of Molecular Cell Biology University Of Occupational And Environmental Health
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Suzuki Minoru
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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MURAKOSHI Masanori
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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Murakoshi M
(present Address)products Strategy Dcpartment Pharma Business Strategy Division Teikoku Hormone Mfg.
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Suzuki M
Division Of Material Science Graduate School Of Environmental Earth Science Hokkaido University
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Murakoshi Masanori
Safety Res. Dept. Teikoku Hormone Mfg Co. Ltd.
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INADA Rie
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.,
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Inada Rie
Safety Res. Dept. Teikoku Hormone Mfg Co. Ltd.
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Mikami T
Department Of Drug Safety Research Eisai Kawashima Research Laboratories Eisai Co. Ltd.
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Suzuki Minoru
Safety Resarch Department Teikoku Hormone Mfg Co. Ltd.
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Watanabe Katsuto
Department Of Pathology School Of Medicine Tokai University
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Ikada Rie
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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