Polyclonal Anti-Desmosine Antiserum Is Specific to Desmosine Molecule : No Cross-Reaction to Pyridinoline
スポンサーリンク
概要
- 論文の詳細を見る
Inhibition ELISA assay was used to examine the cross-reaction of the polyclonal anti-desmosine antiserum produced in rabbit against molecules possessing a "pyridinium ring" as their core structure i.e. isodesmosine, pentasine, pyridinoline and 2'-deoxypyridinoline, highly purified with column chromatography, and structurally unrelated substances, i.e. cysteic acid, taurine and 2-aminopyridine (core structure of desmosine). No cross-reaction was observed to the pyridinoline, 2'-deoxypyridinoline possessing "pyridinium ring" and derived from collagen cross-links, structurally unrelated cysteic acid and taurine, nor core structure of 2-aminopyridine. The antiserum specifically recognized the molecules derived from the elastin cross-links. Using the ELISA assay system with antisera and amino acids analysis, 10μg of desmosine were extracted from 1.0mg of the hydrolysate of commercial elastin.
- 東海大学の論文
著者
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Watanabe K
Aoba Gakuen
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WATANABE Toshiteru
Cell Biology Research Laboratory, School of medicine, Tokai University
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MATSUKI Hideaki
Department of Nursing, School of Health Sciences, Tokai University
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KASUGA Hitoshi
Department of Public Health, School of Medicine, Tokai University
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Watanabe Toshiteru
Cell Biology Research Laboratory
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Kasuga Hitoshi
Tokai University
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Kasuga H
Tokai University School Of Medicine
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Kasuga Hitoshi
Department Of Public Health School Of Medicine Tokai University
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OGAWA Tadao
Laboratory of Biochemistry, Department of Chemistry, Kitasato University
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KAWANISHI Yasuhiro
Laboratory of Biochemistry, Department of Chemistry, Kitasato University
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Ogawa T
Laboratory Of Biochemistry Department Of Chemistry Kitasato University
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Matsuki H
Tokai University Junior College Of Nursing And Medical Technology.
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Matsuki Hideaki
Department Of Nursing School Of Health Sciences Tokai University
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Kawanishi Y
Ogata Inst. Medical And Chemical Res. Chiyoda‐ku
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Kawanishi Yasuhiro
Laboratory Of Biochemistry Department Of Chemistry Kitasato University
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