Glutathione-Peroxidase (GSH-PO) and Androgen Action in Prostate : An Immunohistochemical Study
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概要
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The effect of testosterone and estradiol on the prostate of castrated rats (rat prostatic hyperplasia model after combined treatment with testosterone and estradiol) were investigated by histopathologically and immunohistochemical procedures. The castrated rats were treated for 6 weeks with 1) testosterone 1 mg/animal and 2) testosterone 1 mg/ animal plus 17beta-estradiol (estradiol) 10 jag/animal. Histopathologically, glandular hyperplasia of the ventral prostate and glandular hyperplasia with fibromuscular stromal proliferation in the dorsolateral prostate, was clearly observed. Immuno- histochemical localization of glutathione peroxidase (GSH-PO) which effectively reduces the lipid peroxides, was predominantly observed in the glandular epithelial cells of the ventral prostate. The intensity of GSH-PO staining in the glandular epithelial cells was remarkably decreased after castration, and that it was clearly recovered by testosterone or testosterone plus estradiol treatment to the castrated rats. In contrast, GSH-PO was clearly observed in the glandular epithelial cells of the dorsolateral prostate following testosterone alone or testosterone plus estradiol. Androgen receptor (AR) is mainly localized in nuclei. In the dorsolateral prostate, AR was also detected in the nuclei of the proliferated stromal fibromuscular cells. Furthermore, immunodetectable AR rapidly declined after androgen withdrawal and returned to intact levels of staining intensity after androgen replacement. These findings strongly suggest that expressions of GSH-PO and AR in the prostate are testosterone-dependent. It is concluded that immunohistochemical analysis, using GSH-PO and AR, may be a useful method for prediction of the effects of androgen action on the rat prostate.
- 日本組織細胞化学会の論文
著者
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OSAMURA R.
Department of Pathology, Tokai University School of Medicine
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Osamura R.yoshiyuki
東海大学 医学研究科病理病態学
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Watanabe Toshiteru
Research Laboratory School Of Medicine Tokai University
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Watanabe K
Aoba Gakuen
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Osamura Robert
Dep. Of Pathology Tokai Univ. School Of Medicine
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Osamura R.
Department Of Pathology School Of Medicine Tokai University
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Watanabe Keiichi
Aoba Gakuen Junior College
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Miyazaki Masanobu
Miyazaki clinic
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Osamura R
Tokai Univ. School Of Medicine Kanagawa Jpn
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Osamura R.yoshiyuki
Department Of Pathology Tokai University School Of Medicine
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MURAKOSHI Masanori
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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Murakoshi M
(present Address)products Strategy Dcpartment Pharma Business Strategy Division Teikoku Hormone Mfg.
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Miura Masahiko
Department Of Internal Medicine School Of Medicine Tokai University
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Osamura R.
Tokai Univ. School Of Medicine Kanagawa Jpn
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Murakoshi Masanori
Safety Res. Dept. Teikoku Hormone Mfg Co. Ltd.
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Osamura R
Tokai Univ. School Of Medicine
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Watanabe K.
Dept. Obst. And Gynec. St. Marianna Univ. Sch. Med.
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Watanabe Keiko
Department Of Pathology And Biodefense Division Of Cellular And Molecular Pathology Faculty Of Medic
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Watanabe K.
Dept.of Phthology Tokai Univ.sch.of Med. : Aoba College
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Watanabe Katsuto
Department Of Pathology School Of Medicine Tokai University
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