Isolation of Parietal Cells from Guinea-pig Gastric Mucosa by a Percoll Density Gradient
スポンサーリンク
概要
- 論文の詳細を見る
Isolation of parietal cells from guinea-pig gastric mucosa was performed by density gradient centrifugation using percoll, a new gradient medium composed of colloidal silica coated with polyvinylpyrolidone. The gastric mucosa of the oxyntic gland area was exfoliated, minced and digested with 2.0mg/ml of dispase and 0.1mg/ml of collagenase. Dispersed mucosal cells were applied to the percoll density gradient. Parietal cells were fractionated in the layer between 1.04 and 1.06g/ml in density with purity of 80〜90%. For identification of parietal cells staining with tetranitroblue-tetrazolium and electron microscopy were used in addition to common staining. The viability of the cells was over 95% assessed by trypan-blue exclusion. The parietal cells obtained responded actively to histamine stimulation when ^<14>C-aminopyrine uptake was used as an functional index. In conclusion, percoll density gradient medium is suitable for yielding isolated parietal cells with both high purity and high viability.
- 東海大学の論文
著者
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Miwa Takeshi
Department Of Internal Medicine School Of Medicine Tokai University
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HARA Masafumi
Department of Internal Medicine, School of Medicine, Tokai University
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TANI Norio
Department of Internal Medicine, Shcool of Medicine, Tokai University
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Watanabe Yoriko
東海大学 第6内科
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Watanabe Toshiteru
Research Laboratory School Of Medicine Tokai University
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Watanabe K
Aoba Gakuen
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Watanabe Keiichi
Department Of Internal Medicine School Of Medicine Tokai University
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Tamaoki N
Central Inst. For Experimental Animals
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Tani Norio
Department Of Internal Medicine School Of Medicine Tokai University
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Tani N
The 6th Department Of Internal Medicine School Of Medicine Tokai University
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Tani Norio
6th Department Of Internal Medicine School Of Medicine Tokai University
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Tani Norio
The 6th Department Of Internal Medicine School Of Medicine Tokai University
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Miura T
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Miwa T
The 6th Department Of Internal Medicine School Of Medicine Tokai University
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WATANABE Yoriko
Research Laboratory, School of Medicine, Tokai University
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KARASAWA Hiroyuki
Department of Internal Medicine, School of Medicine, Tokai University
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Tamaoki Norikazu
Central Inst. For Experimental Animals
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Mitomi Toshio
Second Department Of Surgery Tokai University School Of Medicine
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Miwa Takeshi
The 6th Department Of Internal Medicine School Of Medicine Tokai University
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Hara Masafumi
Department Of Internal Medicine School Of Medicine Tokai University
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Watanabe Yoriko
Research Laboratory School Of Medicine Tokai University
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Miwa Takeshi
6th Department Of Internal Medicine School Of Medicine Tokai University
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Karasawa Hiroyuki
Department Of Internal Medicine School Of Medicine Tokai University
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Miwa Takeshi
Department Of Internal Medicine 6 Tokai University School Of Medicine
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Watanabe Keiichi
Department Of Pathology School Of Medicine Tokai University
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Watanabe Keiichi
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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WATANABE Keiichi
Department of Pathology, School of Medicine, Tokai University
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