Production and Properties of Mouse Monoclonal Anti-Adriamycin Antibody
スポンサーリンク
概要
- 論文の詳細を見る
Eight mouse 1gG_1 monoclonal anti-Adriamycin antibodies were produced in culture and in ascites in BALB/c nude mice. The binding constant and specificity was measured by an inhibition ELISA method. The assay was an indirect method using horseradish peroxidase-labeled goat Fab' antibody and 2mM ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) containing 2.5mM H_2O_2 as a color reaction reagent. The binding constant of the antibodies was 10^6 to 10^8 M^<-1>. The antibodies possessed different specificities and affinities to the adriamycin. Though adriamycin is a low molecular weight (M. W. 579.99) hapten antigen, it possesses several epitopes as antigenic determinant sites. The antigenic epitopes of the adriamycin, which until recently were unknown, were analyzed by the monoclonal antibodies.
- 東海大学の論文
著者
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MITOMI Toshio
Department of Surgery, School of Medicine, Tokai University
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Watanabe K
Aoba Gakuen
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Mitomi T
Tokai Univ. School Of Medicine
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NAKASAKI Hisao
Department of General Surgery, School of Medicine, Tokai University
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KASUGA Hitoshi
Department of Public Health, School of Medicine, Tokai University
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Kasuga Hitoshi
Tokai University
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Kasuga H
Tokai University School Of Medicine
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Kasuga Hitoshi
Department Of Public Health School Of Medicine Tokai University
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Nakasaki H
Department Of Surgery Tokai University School Of Medicine
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Nakazaki Hisao
Department Of Pathology School Of Medicine Tokai University
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MATSUKI Hideaki
Tokai University Junior College of Nursing and Medical Technology
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WATANABE Toshiteru
Laboratories for Structure and Function, Tokai University School of Medicine
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KAMIJOU Akemi
Department of Surgery, Tokai University School of Medicine
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Mitomi T
Department Of Surgery Tokai University School Of Medicine
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Mitomi Toshio
Department Of 2nd Surgery School Of Medicine Tokai University
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Kamijou Akemi
Department Of Surgery Tokai University School Of Medicine
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Matsuki H
Tokai University Junior College Of Nursing And Medical Technology.
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Watanabe Katsuto
Department Of Pathology School Of Medicine Tokai University
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Nakasaki H
Department Of Surgery Tokai University Oiso Hospital
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