Purification, Properties and Identification of a Serum DNA Binding Protein (64DP) and Its Microheterogeneity
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概要
- 論文の詳細を見る
A DNA binding protein with a molecular weight of 64,000, designated 64DP, has been purified and characterized. This protein was isolated from adult human pooled serum by DEAE Sephadex column Chromatography, DNA cellulose affinity column chromatography, ammonium sulfate fractionation and Sephadex G-150 gel filtration. The final preparation of 64DP was homogeneous, as judged from polyacrylamide gel electrophoresis with or without sodium dodecyl sulfate and sedimentation experiments. Physicochemical and immunochemical properties of this protein were very similar or identical to those of a-1-antichymotrypsin with some differences in electric mobility and the pattern of isoelectric focusing. Furthermore, the general properties of 64DP from various sera were practically similar with the exception that isoelectric focusing analysis showed microheterogeneity among 64DP purified from various sera.
- 東海大学の論文
著者
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Tsuda Michio
Department of Medical Education Tokai University, School of Medicine
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MITOMI Toshio
Department of Surgery, School of Medicine, Tokai University
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NAKASAKI Hisao
Department of General Surgery, School of Medicine, Tokai University
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Tsuda Michio
Department Of Biochemistry School Of Medicine Tokai University
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KATSUNUMA Tsunehiko
Department of Biochemistry and Microbiology, School of Medicine Tokai University
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SUZUKI Kazumi
Departments of Urology, Jichi Medical School
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Katsunuma Tsunehiko
Department Of Biochemistry School Of Medicine Tokai University
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Tamura M
Univ. Tsukuba Ibaraki
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Nakasaki Hisao
Department Of Surgery School Of Medicine Tokai University
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OHKUBO Tomoichi
Department of Biochemistry, Tokai University
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Ohkubo Tomoichi
Department Of Biochemistry Tokai University
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Suzuki Kazumi
Department Of Urology Jichi Medical School
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KAMIGUCHI Hiroshi
Division of Biochemistry, Interdepartmental Laboratory, School of Medicine, Tokai University
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Mitomi Toshio
Department Of 2nd Surgery School Of Medicine Tokai University
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Kamiguchi Hiroshi
Division Of Biochemistry Interdepartment Laboratory School Of Medicine Tokai University
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Kamiguchi Hiroshi
Division Of Biochemistry Interdepartment Laboratory
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Ohkubo T
Department Of Biochemistry Tokai University
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Ohkubo Tomoichi
Department Of Biochemistry School Of Medicine Tokai University
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Suzuki Kazumi
Department Of Biochemistry School Of Medicine Tokai University
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KATSUNUMA Tsunehiko
Department of Biochemistry, School of Medicine, Tokai University
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