Peripheral Blood Neutrophil Function in Germfree and Conventional Rats
スポンサーリンク
概要
- 論文の詳細を見る
The superoxide anion production and the myeloperoxidase activity between polymorphonuclear cells (PMNs) from conventional and germfree rats were compared. Furthermore, the response to recombinant human granulocyte colony stimulating factor (G-CSF) between both groups of rats was also examined. Our present investigation showed that: (1) the basal superoxide anion production by PMNs in germfree rats (2.1±0.5 nmol/min/1×10^6 PMNs) is less than one fourth of that of conventional rats (9.5±2.9 nmol/min/1×10^6 PMNs). (2) the myeloperoxidase activity of PMNs from germfree rats (38.1±5.2 nmol/min/1×10^6 PMNs) is 1.7 times that of conventional rats (22.4±4.9 nmol/min/1×10^6 PMNs). (3) G-CSF administration increased the number of PMN, 12.4 times in the germ free rats compared with 3.9 times in the conventional rats. (4) the PMNs induced by G-CSF do not differ in the superoxide anion producing activity. However the myeloperoxidase activity decreased in the G-CSF induced PMNs of both groups of rats. From our data, the conventional rats could have a more effective killing activity than the germfree rats. Furthermore, germfree rats may be used as a powerful tool for assay of other biological substances relating to immune system and its regulation.
- 東海大学の論文
- 1990-03-08
著者
-
Ogoshi Kyoji
Department of Gastroenterological Surgery, Tokai University
-
Tsuda Michio
Department of Medical Education Tokai University, School of Medicine
-
Ozawa Atsushi
Department of Cardiology, Yokohama City Seibu Hospital, St. Marianna University
-
Ogoshi Kyoji
Department Of Surgery Tokai University
-
Tsuda Michio
Department Of Biochemistry School Of Medicine Tokai University
-
Ohnishi Nobuhiko
Department of Cardiology, Mitoyo General Hospital
-
YAMAMURA Masaichi
Department of Molecular Life Science, Tokai University School of Medicine
-
Tamura M
Univ. Tsukuba Ibaraki
-
Tamura M
Hokkaido Univ.
-
Ozawa Atsushi
Department Of Cardiology St. Marianna University Yokohama City Seibu Hospital
-
OHKUBO Tomoichi
Department of Biochemistry, Tokai University
-
TAMURA Mariko
Division of Biochemistry of Interdepartment Laboratory, Tokai University
-
Ohkubo Tomoichi
Department Of Biochemistry Tokai University
-
Ohnishi Nobuhiko
Department Of Cardiology Mitoyo General Hospital
-
Tamura Mariko
Division Of Biochemistry Of Interdepartment Laboratory Tokai University
-
Yamamura Masaichi
Department Of Biochemistry School Of Medicine Tokai University
-
Ohnishi Nobuhiko
Department Of Microbiology School Of Medicine Tokai University
-
Ohkubo Tomoichi
Department Of Biochemistry School Of Medicine Tokai University
-
Ozawa Atsushi
Department Of Bacteriology 2nd National Tokyo Hospital
関連論文
- HLA-B54 is a candidate of response to Fluoropyrimidine plus PSK therapy in gastric cancer
- OE-337 Activin A can Induce the Differentiation of Cardiomyocytes from Medaka Embryonic Cells in vitro(Development and differentiation(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-533 Relationship between Smoking Cassation and Habitual Exercise in Patients with Ischemic Heart Disease(Smoking 2 (H) : PJ89)(Poster Session (Japanese))
- PJ-530 Study on lifestyle habits and oxidative stress in patients with coronary diseases(Smoking 2 (H) : PJ89)(Poster Session (Japanese))
- OJ-493 Medico-economic impacts of exercise habits on the drug therapy for hyperlipidemia in the secondary prevention of ischemic heart disease(Exercise Test/Cardiac Rehabilitation 8 (IHD) : OJ60)(Oral Presentation (Japanese))
- OJ-492 Long-term prognosis of patients with coronary disease who underwent PCI followed by exercise therapy(Exercise Test/Cardiac Rehabilitation 8 (IHD) : OJ60)(Oral Presentation (Japanese))
- Long term outcomes comparing between endscopic mucosal resection and traditional gastrectomy for treatment of early gastric cancer
- Alpha-1-Antichymotrypsin (ACT) as an Immune Regulatory Agent
- The Possibility of Fabricating Co-Cr Casting Plates by Using a Heat-Shock Method with Conventional Phosphate-Bonded Investments
- Two-stage Heat-Shock Procedure Can Fabricate Sound Co-Cr Casting Denture Plates by Using Conventional Phosphate-Bonded Investments
- Septicemia during Compromised Host Treated in a Protected Isolation Unit
- Bacteriological Evaluation of an Isolation Ward during Patient Occupancy
- The Utility of Protective Environment and Prophylactic Antibiotics in the Therapy of Leukemia
- The Level of Purification and Sterilization Methods in the Bioclean Room
- A low cost and quick assay system using the free-living nematode Caenorhabditis elegans to determine the effects of Kampo medicines on life span
- The Effects of the HMG-CoA-RI against Restenosis Following Coronary Stent Implantation
- Evaluation of Time-Sequence Changes in Whole Blood Platelet Function Following Percutaneous Coronary Intervention with Stent Implantation
- The Relationship between Host and Intestinal Flora : 2. Studies on Changes in the Amounts of Amino Acids in Intestinal Contents among Conventional, Antibiotic-treated and Clostridium butyricum-infected Rats.
- The Relationship between Host and Intestinal Flora : I. Studies on Changes in the Kinds of Intestinal Bacterial Flora and Concentration of Amino Acids in Various Organs of Rats in Accordance with Various Dietary Conditions
- The Effect of Amino Acid Infusion on Anesthesia-Induced Hypothermia in Muscle Atrophy Model Rats
- PE-477 Significant Increase of Cholesterol, Trigriceride and AA were Observed in AMI : From 20 Years Database in Mitoyo General Hospital(PE080,ACS/AMI (Clinical/Pathophysiology/Treatment/Basic), Angina Pectoris (Clinical) (IHD),Poster Session (English),Th
- PE-363 Difference of Risk Factors and PUFAs between Generations and Sex in Patients of Acute Myocardinal Infarction(PE061,ACS/AMI (Clinical/Pathophysiology) 2 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation So
- PE-203 QT Prolongation and Global ST-T Change are the Characteristics of Electrocardiogram of Heat Stroke(PE034,ECG/Body Surface Potential Mapping/Holter (A),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Serum N-3 Polyunsaturated Fatty Acid Levels Correlate With the Extent of Coronary Plaques and Calcifications in Patients With Acute Myocardial Infarction
- Congenital Giant Aneurysm of the Left Atrial Appendage Mimicking Pericardial Absence : Case Report
- A Case of Gastrointestinal Stromal Tumor of the Stomach
- Immunological Considerations in Patients with Tonsillar Lesions
- A Case for Gastrointestinal Stromal Tumor (GIST) with Reference to Its Ultrastructure and 'Gain-of-Function' Mutation
- Preliminary Study for Sentinel Lymph Node Identification with Tc-99m Tin Colloid in Patients with Esophageal or Gastric Cancer
- PJ-475 Coronary Vessel Study by 16-row MDCT Could Feasibly Identify Vulnerable Plaques : Comparison to Virtual Histology IVUS Images(CT/DSA-10, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-172 A Ratio of AA to EPA is More Strongly Related to the Prognosis after AMI than Other Coronary Risk Factors(Acute myocardial infarction, clinical (diagnosis/treatment)-9, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-072 Serum Antibodies to Human Heat Shock Protein 60 Is Elevated in Subjects With Unstable Angina Pectoris(Acute Coronary Syndrome, Basic/Clinical 3 (IHD) : OE9)(Oral Presentation (English))
- Investigation of the Long-Term Training Effect on Patients with Myocardial Infarction
- Impact of Routine Exercise on Medical Costs in Patients with Ischemic Heart Disease
- Regulation of Heat Production of Brown Adipocytes via Typical and Atypical β-Adrenoceptors in the Rat
- Effect of Beta-Blocker Therapy on Autonomic Activity in Patients with Dilated Cardiomyopathy
- Exercise compliance in the long term in patients with ischemic heart disease
- Gastrointestinal Cytomegalovirus Infection in Collagen Diseases
- Laparoscopy-Assisted Combined Resection for Synchronous Gastric and Colorectal Cancer : Report of Three Cases
- Binding of Modified Alpha-1-Antichymotrypsin to Mitogen-Stimulated Human Lymphocyte Membrane : A Model for Immune Suppression
- Incorporation of Alpha-1-Antichymotrypsin into Human Stomach Adenocarcinoma Cell Nuclei and Inhibition of DNA Primase Activity
- Evaluation of Prognoses of Patients with Gastric Cancer by the Responsiveness of Peripheral Blood Lymphocyte to OK432 in vitro
- Clinical Significance of Serum Soluble HLA Class I Antigens in Systemic Lupus Erythematosus
- A Case of Superficial Esophageal Cancer Complicated with Idiopathic Muscular Hypertrophy of the Esophagus
- PJ-527 AA/EPA is a Strong Indicator of Plaque Vulnerability in Both ACS and SAP : A Study with VH-IVUS(PJ089,Atherosclerosis (Clinical/Pathophysiology) 1 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Societ
- Protein Tyrosine Phosphatase PTPεM Induces Apoptosis in Porcine Ovarian Granulosa Cells
- Peripheral Blood Neutrophil Function in Germfree and Conventional Rats
- Correlation of Mitogen-Activated Protein Kinase Activities with Cell Survival and Apoptosis in Porcine Granulosa Cells(Reproductive Biology)
- Isolation, Purification and Identification of a Serum DNA Binding Protein which Increases in Malignant Diseases
- Alpha 1-Antichymotrypsin and IgM as DNA-binding Protein in Serum : A Possible Diagnosis for Malignant Ovarian Tumors
- Hemolytic Streptococcus Preparation OK-432; Benefical Adjuvant Therapy in Recurrent Gastric Carcinoma
- Effects of Oral Administration of Streptococcal Preparation OK-432 on Thoracic Duct Lymphocytes
- Effects of Biological Response Modifier on Thoracic Duct Lymphocytes in Recurrent Gastric Cancer Evaluation of OK-432, a Hemolytic Streptococcus Preparation
- Effect of Recombinant Human Granulocyte Colony-Stimulating Factor (rhG-CSF) on Allogeneic Bone Marrow Transplantation in Mice
- Gastrointestinal Decontamination in the Compromised Host and its Clinical Significance
- Arachidonic Acid/Eicosapentaenoic Acid is Strongly Related to Soft Plaque Volume Assessed by MDCT in Acute Myocardial Infarction(Atherosclerosis, Clinical 4 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- A Left Ventricular Function Evaluated by ECG Gated MDCT Heart Scan is Compatible to a Left Ventriculography(X-ray/CT/MRI/DSA 5 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- MDCT could Feasibly Predict not only Subacute but also Remote Phase Left Ventricular Function after Acute Myocardial Infarction(X-ray/CT/MRI/DSA 9 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-272 MDCT Could not only Create Three Dimensional Images of Septum but also Qantifiy Qp/Qs of Atrial Septal Defect(X-ray/CT/MRI/DSA5 (I) : PJ46)(Poster Session (Japanese))
- PE-176 Myocardial Damage Detected by MDCT Could Predict Functional Recovery More Accurately than Tl or BMIPP SPECT in Acute Anteroseptal Infarction(X-ray/CT/MRI/DSA6 (I) : PE30)(Poster Session (English))
- FRS-067 The Ratio of Arachidonic Acid to Eicosapentaenoic Acid is Important for Formation of Multiple Complex Plaques in Acute Myocardial Infarction(Atherosclerosis (IHD) : FRS8)(Featured Research Session (English))
- Effects of Dopamine and Substance P Infusion on Gastric Acid Secretion in Pavlov and Heidenhain Pouched Dogs
- Concentrations of Alpha-1-antichymotrypsin and Other Acute Phase Reactants in Patients with Gastric Cancer
- Purification, Properties and Identification of a Serum DNA Binding Protein (64DP) and Its Microheterogeneity
- Influences of Low Intensity Exercise on Body Composition, Food Intake and Aerobic Power of Sedentary Young Females
- Demonstration of HCG on the Surface of Maternal Lymphocytes and Discrimination of T and B Cells by Esterase Cytochemistry
- α-1-Antichymotrypsin gene A1252G variant (ACT Isehara-1) is associated with a lacunar type of ischemic cerebrovascular disease
- Suppression of Deoxyguanosine Cytotoxicity and Deoxyguanosine Kinase Activity in Mouse FM3A Mammary Carcinoma Cell : Mutants Defective in Hypoxanthine Phosphoribosyl-transferase
- Labor Reduction for Mold Preparation of a Commercial Titanium Cast Denture System Using a Heat-shock Method
- Ecological Investigation on Intestinal Bacterial Flora of Patients Nursed in Protected Environmental Units
- The Germfree Ward of Tokai University Hospital
- The Isoelectric Focusing Pattern of Desialylated α1-antichymotrypsin of Heterozygous α1-antichymotrypsin Deficiency and of Acute Phase Plasma
- Ecological Mechanism of Protection of Intestinal Bacterial Flora Against Salmonella typhimurium Infection
- Intestinal Bacterial Flora and Host Defense Mechanisms
- Experimental study on duodenal food passage vs. non-duodenal food passage reconstruction
- B-1. Immunological Study in Brain Tumor : Immunoserological Survey of Pstients with Brain Tumor
- Behavior of Cell-Mediated Immune Functions and Humoral Factors in Cancer Patients
- Congenital Absence of the Left Circumflex Coronary Artery Associated With Acute Myocardial Infarction : A Case Report
- Detection of Herpes Simplex DNA in Semen and Menstrual Blood of Individuals Attending an Infertility Clinic
- Heat Production as a Quantitative Parameter for Cell Differentiation and Cell Function
- The Role of the Brain in Immune Response in Rats
- Comparison of Argininosuccinate Synthetase from Young and Old Rat Livers
- Heat Production due to Intracellular Killing Activity
- Focus on the frequencies of serological HLA antigens in 4,094 Japanese people
- HLA-D/DR Linked Immune Response to Candida Allergen : An in vivo and in vitro Study in Japanese
- Nonfunctioning Pancreatic Endocrine Tumor with Extension into the Main Pancreatic Duct : Report of a Case
- Selection of Hypotoxigenic Vibrio cholerae Population by Human Intestinal Bacteria in Gnotobiotic Mice
- Molecular Properties of Cholera Enteroxin
- Inhibition of DNA Synthesis by α-1-Antichymotrypsin
- Host use in relation to food availability and larval development in the specialist herbivore Epilachna admirabilis (Coleoptera : Coccinelidae)
- Effective and ineffective personalized therapy based on serum HLA from a 30-year odyssey
- A case of severe biliary stenosis after cholecystectomy and hepatoduodenal ligament lymph node dissection for early gallbladder cancer
- Attenuated expression of menin and p27^ in an aggressive case of multiple endocrine neoplasia type 1 (MEN1) associated with an atypical prolactinoma and a malignant pancreatic endocrine tumor
- An annular pancreas associated with carcinoma of the papilla of Vater : report of a case
- Impact of Chronic Kidney Disease on Left Main Coronary Artery Disease and Prognosis in Japanese Patients
- Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats
- Which is better long-term survival of gastric cancer patients with Billroth I or Billroth II reconstruction after distal gastrectomy? -Impact on 20-year survival rate-
- Preoperative body mass index and outcomes of gastric cancer patients
- Outcome of HLA-oriented therapy for gastric cancer in retrospective and prospective study.
- Adjuvant therapy with imatinib mesylate after resection of primary high-risk gastrointestinal stromal tumors in Japanese patients