Incorporation of Alpha-1-Antichymotrypsin into Human Stomach Adenocarcinoma Cell Nuclei and Inhibition of DNA Primase Activity

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概要

• 論文の詳細を見る

• Incorporation of alpha-1-antichymotrypsin (ACT) into human stomach adenocarcinoma cell nuclei and the effect of ACT on DNA primase from the same carcinoma cells were studied. ACT or [^<125>I]-ACT were observed in carcinoma cell nuclei and high specific radioactivity was detected in washed nuclear fraction when 0.4mg of ACT or [^<125>I] ACT (8×10^7cpm) was intravenously injected into carcinoma bearing nude mice 2h before killing. The molecular weight of radioactivity presented in cell nuclei was same as the intact ACT on SDS-polyacrylamide gel electrophoresis. ACT inhibited DNA primase activity and this inhibiting activity was stable than its chymotrypsin inhibiting activity. The results presented here show ACT is incorporated into carcinoma cell nuclei without modification of its molecular weight and may inhibit DNA primase activity.

• 東海大学の論文
• 1988-03-17

著者

• MATSUMOTO Masahiko

  Department of Biochemistry, School of Medicine, Tokai University

• Tsuda Michio

  Department of Medical Education Tokai University, School of Medicine

• Takada Shigeo

  東海大学 生化

• Takada Shigeo

  Department Of Biochemistry School Of Medicine Tokai University

• Tsuda Michio

  Department Of Biochemistry School Of Medicine Tokai University

• KATSUNUMA Tsunehiko

  Department of Biochemistry and Microbiology, School of Medicine Tokai University

• YAMAMURA Masaichi

  Department of Molecular Life Science, Tokai University School of Medicine

• FUJINAMI Sadako

  Department of Biochemistry, School of Medicine, Tokai University

• Katsunuma Tsunehiko

  Department Of Biochemistry School Of Medicine Tokai University

• Matsumoto Masahiko

  Department Of Biochemistry School Of Medicine Tokai University

• Fujinami Sadako

  Department Of Biochemistry School Of Medicine Tokai University

• Tamura M

  Univ. Tsukuba Ibaraki

• Yamamura Masaichi

  Department Of Biochemistry School Of Medicine Tokai University

• TAKADA Shigeo

  Department of Biochemistry, School of Medicine, Tokai University

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