Binding of Modified Alpha-1-Antichymotrypsin to Mitogen-Stimulated Human Lymphocyte Membrane : A Model for Immune Suppression
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概要
- 論文の詳細を見る
Alpha-1-antichymotrypsin (ACT), an acute phase reactant protein elevated during acute inflammation, and its derivatives (asialo ACT and acid-exposed asialo ACT) were investigated their effect on lymphocyte proliferative responses, and evidence for binding to lymphocyte membrances as well as the characteristics of this binding were investigated. Acid-exposed asialo ACT significantly reduced ^3H-thymidine incorporation into human peripheral lymphocytes stimulated by phytohemagglutinin (PHA) though native ACT could not inhibit the mitogen-induced lymphoproliferation and asialo ACT moderately inhibited it. In order to determine the interaction of ACT and its derivatives to lymphocyte membranes, the binding of ^<125>I-labelled ACT and its derivatives to membranes of intact lymphocyte and extracted lymphocyte membranes was examined. The binding of ^<125>I-labelled native ACT and asialo ACT to resting and PHA-stimulated lymphocyte membrane was low. And the binding of ^<125>I-labelled acid-exposed asialo ACT to resting lymphocyte membrane was also low. However, when lymphocytes were stimulated by mitogens the binding of ^<125>I-labelled acid-exposed asialo ACT increased significantly. The binding of ^<125>I-labelled acid-exposed asialo ACT to the membrane extracted from PHA-stimulated lymphocytes was time-dependent and saturation was reached at 120min at 37℃. One mg of membrane could bind a maximum of approximately 83 pmol of acid-exposed asialo ACT with dissociation constant of 0.73μM. Other unlabelled serum glycoproteins such alpha-1-antitrypsin, alpha-1-acid glycoprotein, transferrin, and proteinase inhibitors including chymostatin, leupeptin and soy bean trypsin inhibitor did not compete with ^<125>I-labelled acidexposed asialo ACT for binding sites in simultaneous competition assays.
- 東海大学の論文
- 1988-03-17
著者
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MATSUMOTO Masahiko
Department of Biochemistry, School of Medicine, Tokai University
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Takada Shigeo
東海大学 生化
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Takada Shigeo
Department Of Biochemistry School Of Medicine Tokai University
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Nakamura Kenji
Department of Surgery, Tokai University School of Medicine
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KATSUNUMA Tsunehiko
Department of Biochemistry and Microbiology, School of Medicine Tokai University
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YAMAMURA Masaichi
Department of Molecular Life Science, Tokai University School of Medicine
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NAKAMURA Kazue
Department of Biochemistry, School of Medicine, Tokai University
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Katsunuma Tsunehiko
Department Of Biochemistry School Of Medicine Tokai University
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Matsumoto Masahiko
Department Of Biochemistry School Of Medicine Tokai University
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Nakamura K
Department Of Surgery Tokai University School Of Medicine
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Yamamura Masaichi
Department Of Biochemistry School Of Medicine Tokai University
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TAKADA Shigeo
Department of Biochemistry, School of Medicine, Tokai University
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