Demonstration of HCG on the Surface of Maternal Lymphocytes and Discrimination of T and B Cells by Esterase Cytochemistry

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概要

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• Immunological functions pregnant woman in the early fetomaternal relationship were investigated. Maternal T cell population as determined by the standard method gradually decreases as plasma HCG increases from between 5 and 10 weeks of gestation. In this study, the presence of HCG on the surface of maternal lymphocytes was detected by the direct immuno-histochemical method using enzyme-labeled antibodies: (1) HRP-anti human β-HCG rabbit Fab', (2) HRP-anti α-HCG rabbit Fab', (3) HRP-anti human native HCG rabbit Fab'. Further (4) HRP-normal rabbit Fab', (5) HRP-anti rabbit IgG goat F (ab')_2 and (6) activated HRP were used as staining controls. In order to determine whether the maternal lymphocytes masked with HCG were T or B cells, a modified technique of esterase cytochemistry was employed. The reaction of esterase cytochemistry on rosette forming lymphocytes by SRBC exhibited the features of T cells, as the lymphocytes display a dense localized positivity made up of one to four coarse granules.

• 東海大学の論文

著者

• Ozawa Atsushi

  Department of Cardiology, Yokohama City Seibu Hospital, St. Marianna University

• Watanabe Keiichi

  Department Of Internal Medicine School Of Medicine Tokai University

• YAMAUCHI Susumu

  Division of Clinical Microbiology, School of Medicine, Tokai University

• Izumi Shinichi

  Cell Biol. Res. Lab. Tokai Univ. Sch. Med.

• Ozawa Atsushi

  Department Of Cardiology St. Marianna University Yokohama City Seibu Hospital

• Izumi Shinichi

  Cell Biology Research Laboratory School Of Medicine Tokai University

• SHIOTSUKA Yukihiko

  Department of Obstetrics and Gynecology, School of Medicine, Tokai Universtiy

• Shiotsuka Yukihiko

  Department Of Obstetrics And Gynecology School Of Medicine Tokai University

• Yamauchi Susumu

  Division Of Clinical Microbiology School Of Medicine Tokai University

• Watanabe Keiichi

  Department Of Pathology School Of Medicine Tokai University

• Watanabe Keiichi

  Department Of Applied Biological Science Faculty Of Agriculture Saga University

• Ozawa Atsushi

  Department Of Bacteriology 2nd National Tokyo Hospital

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