Measurement of Cross-Reactive Properties of Adriamycin Derivatives by the Inhibition Enzyme-Linked Immunosorbent Assay for Adriamycin
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概要
- 論文の詳細を見る
An inhibition enzyme-linked immunosorbent assay (Inhibition ELISA) system for adriamycin has been established. In this assay system, polyclonal anti-adriamycin antiserum produced in rabbits was used. The assay system showed sensitivity for adriamycin in the range of 0.1-2.5μg/ml (0.53-4.23 nmoles per ml). This assay system was applied to analyze the cross-reactive properties of substances with chemical structures similar to that of adriamycin. The cross-reactive properties of six kinds of adriamycin derivatives (Epirubicin, Daunorubicin, Pirarubicin, Aclarubicin, SM-5887 and MX-2) against anti-adriamycin antiserum were measured. The cross-reactive properties of each derivative against the antiserum differed in accordance with the differences in the position of their functional groups attached to the anthracyclic ring. The antiserum recognized the -epi type of -OH group at the -4' position on sugar chains as well as number of sugar chains attached to the anthracyclic ring, and it also recognized the glycoloyl or acetyl and/or ethyl group at position 9 on the anthracyclic ring. With this assay system, the antigenic determinant sites of the anticancer drugs were inferred.
- 東海大学の論文
著者
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MITOMI Toshio
Department of Surgery, School of Medicine, Tokai University
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Watanabe K
Aoba Gakuen
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Mitomi T
Tokai Univ. School Of Medicine
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NAKASAKI Hisao
Department of General Surgery, School of Medicine, Tokai University
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Nakasaki H
Department Of Surgery Tokai University School Of Medicine
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Nakazaki Hisao
Department Of Pathology School Of Medicine Tokai University
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WATANABE Toshiteru
Division of Cell Biology Research Laboratory, School of Medicine, Tokai University
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Mitomi T
Department Of Surgery Tokai University School Of Medicine
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Mitomi Toshio
Department Of 2nd Surgery School Of Medicine Tokai University
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Watanabe Katsuto
Department Of Pathology School Of Medicine Tokai University
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Nakasaki H
Department Of Surgery Tokai University Oiso Hospital
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