Experience with an Enzyme-Linked Immuno Sorbent Assay for the Quantitation of Urinary Desmosine

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概要

• 論文の詳細を見る

• An enzyme-linked immuno sorbent assay has been set up for the quantitation of the elastin crosslink desmosine. With the assay desmosine could be detected in the range 0.01-10ng (the amount is corresponding to 0.4-400ng/ml) in standard solutions. One hundred percent crossreactivity with isodesmosine was found. The low titer rabbit antiserum used in this inhibition immunoassay was less suitable for the measurement of desmosine in urinary hydrolysates, because a number of unknown urinary substances interfered with the measurement. Interfering substances could not be removed completely with a column purification method. Future research is needed to determine the applicability of the higher titer anti-desmosine antiserum from a second rabbit.

• 東海大学の論文

著者

• WATANABE Toshiteru

  Department of Pediatirics, School of Medicine, Tokai University

• MATSUKI Hideaki

  Department of Nursing, School of Health Sciences, Tokai University

• KASUGA Hitoshi

  Department of Public Health, School of Medicine, Tokai University

• Watanabe Toshiteru

  Department Of Pediatirics School Of Medicine Tokai University

• Kasuga Hitoshi

  Tokai University

• Kasuga H

  Tokai University School Of Medicine

• Kasuga Hitoshi

  Department Of Public Health School Of Medicine Tokai University

• VERPLANKE Anton

  Department of Public Health, School of Medicine, Tokai University

• ISHIMORI Kei

  Department of Public Health, School of Medicine, Tokai University

• Matsuki H

  Tokai University Junior College Of Nursing And Medical Technology.

• Matsuki Hideaki

  Department Of Nursing School Of Health Sciences Tokai University

• Ishimori Kei

  Cell Biology Research Laboratory And Department Of Public Health School Of Medicine Tokai University

• Verplanke Anton

  Cell Biology Research Laboratory And Department Of Public Health School Of Medicine Tokai University

• Watanabe Toshiteru

  Department Of Molecular And Developmental Science Molecular Medicine Research Center The Institute O

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