A Carbon-Centered Radical as a Reaction Species in the DNA Strand-Breakage by D-Glucosamine

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概要

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• Electron spin resonance(ESR) studies using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and sodium 3,5-dibromo-4-nitrosobenzensulfonate (DBNBS) as a spin-trapping agent revealed the formation of both hydroxyl and carbon-centered radical-derived spin adducts in Cu^<2+>-containing 50 mM Tris-HCl buffered solutions (pH 7.1) of D-glucosamine, D-mannosamine, and D-galactosamine, which were previously shown to have the ability to break the single-strand of plasmid pBR322 DNA in a nucleotide sequence-specific manner.HCl-free D-glucosamine has higher DNA breaking activity, and this activity is promoted more by the presence of Cu^<2+> than the original D-glucosamine hydrochloride, exhibits stronger radical signals in the ESR spectrum. It is suggested that D-glucosamine is unstable around neutral pH, being converted into certain intermediate(s) such as a dihydropyrazine compound, which generate(s) carbon-centered radicals, and that, besides the hydroxyl radical, the intermediate(s) is/are responsible for DNA strand breakage.

• 公益社団法人日本薬学会の論文
• 1998-03-15

著者

• Yamaguchi Takuji

  Central Research Laboratory Tsumura & Co.

• Watanabe K

  School Of Life Science Tokyo University Of Pharmacy And Life Science

• Matsumoto Shun-ichiro

  Institute For Drug Discovery Research Yamanouchi Pharmaceutical Co. Ltd.

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• Satoh Kazue

  Analysis Center, School of Pharmaceutical Sciences,Showa University

• YAMAGUCHI Tadatoshi

  Faculty of Pharmaceutical Sciences, Sojo University

• Satoh Kazue

  Analysis Center School Of Pharmaceutical Sciences Showa University

• KASHIGE Nobuhiro

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YAMAGUCHI Tadatoshi

  Department of Hygiene, Miyazaki Medical College

• WATANABE Kenji

  Faculty of Pharmaceutical Sciences, Fukuoka University

• MATSUMOTO Shigenobu

  Department of Biochemistry and Isotope, Tokyo Metropolitan Institute of Gerontology

• YASUDA Masahide

  Cooperative Research Center, Miyazaki University

• Yamaguchi Tokio

  Faculty Of Pharmaceutical Sciences Sojo University

• Matsumoto Shuji

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Okayama University

• Matsumoto Sayaka

  Faculty Of Pharmaceutical Sciences Osaka University

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Yamaguchi T

  Tsumura Res. Laboratories Tsumura & Co.

• Watanabe K

  Department Of Agriculture Tokyo University Of Agriculture

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Yasuda Masahide

  Cooperative Research Center Miyazaki University

• Tadatoshi Yamaguchi

  Faculty Of Hygiene Miyazaki Medical College

• Yamaguchi Tadatoshi

  Faculty Of Pharmaceutical Sciences Sojo University

• Kashige Nobuhiro

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Watanabe Kenji

  Faculty Of Fisheries Kagoshima University

• Kashige Nobuhiro

  Faculty of Pharmaceutical Science, Fukuoka University

• WATANABE Kenji

  Faculty of Agriculture, Gifu University

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