Substrate Diversity of Macrophomate Synthase Catalyzing an Unusual Multistep Transformation from 2-Pyrones to Benzoates
スポンサーリンク
概要
- 論文の詳細を見る
Macrophomate synthase, which we have recently purified, catalyzes an unusual multistep transformation from 5-acetyl-4-methoxy-6-methyl-2-pyrone to 4-acetyl-3-methoxy-5-methyl-benzoic acid (macrophomic acid). To investigate the substrate diversity of the enzyme, 40 analogs of 2-pyrone were prepared and their relative efficiency was examined in the enzymatic conversions. The experimental results reveal the structural requirements of the substrates and the rough size of the enzyme active site, and eliminate the ambiguity caused by contamination by other enzymes in the whole-cell experiments.
- 社団法人日本農芸化学会の論文
- 2000-03-23
著者
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WATANABE Kenji
Department of Kampo Medicine, Keio University, School of Medicine
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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HONMA Mamoru
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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MIE Takashi
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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ICHIHARA Akitami
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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OIKAWA Hideaki
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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MIE Takashi
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Watanabe K
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ichihara A
Hokkaido Univ. Sapporo Jpn
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Mie Takashi
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma Mamoru
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Watanabe K
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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Ichihara Akitami
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Watanabe Kenji
Department Of Gastroenterology Osaka City University Graduate School Of Medicine
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Watanabe Kenji
Department Of Anatomy Fukui Medical School
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Watanabe Keiichi
The Graduate School Of Science And Technology Kobe University
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Watanabe Kenji
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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ICHIHARA Akitami
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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OIKAWA Hideaki
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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