Function of Cupric Ion in the Breakage of pBR322 ccc-DNA by D-Glucosamine(Biological Chemistry)

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The function of Cu^<2+> in the breakage of pBR322 ccc-DNA by D-glucosamine was investigated using agarose gel electrophoresis. The maximal stimulatory effects on the DNA breakage and ・OH production were observed at the same concentration of Cu^<2+> in an aqueous solution of D-glucosamine. An aqueous solution of D-glucosamine containing 1mM of Cu^<2+> generated less O_2^- and H_2O_2 than ・OH. The generation of these active oxygens, either in the presence or absence of Cu^<2+>, was affected little by the addition of DNA. Superoxide dismutase inhibited the DNA-breaking action of D-glucosamine in the absence, but not the presence, of Cu^<2+>. Hydroxyl radical-scavengers inhibited the reaction in the presence as well as the absence of Cu^<2+>. The reaction in the presence of Cu^<2+> was inhibited by d-GMP and a different kind of DNA. An atomic absorption analysis showed that copper binding to pBR322 ccc-DNA was concentration-dependent. Cupric ions were found to bind to the cccDNA, and were found to stimulate the autoxidation of D-glucosamine via generation of active oxygen molecules, including, especially ・OH.
社団法人日本農芸化学会の論文
1990-03-23