Dihydropyrazine Derivatives as a New Type of DNA Strand Breaking Agent

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概要

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• The DNA strand-breaking activity of some dihydropyrazine derivatives, 2,3-dihydro-5,6-dimethylpyrazine (3), 2,3-dihydro-2,5,6-trimethylpyrazine (4), 2,3-dihydro-2,2,5,6-tetramethylpyrazine (5), trans-2,3-dimethyl-5,6,7,8,9,10-hexahydroquinoxaline (6), its cis-compound (7) and the mixture of 6 and 7 (8) was tested by agarose gel electrophoresis using plasmide pBR322 ccc-DNA as a substrate. The order of DNA strand-breaking activity in the presence of Cu^<2+> was (7)>(8)≧(5)>(2)>(6)>(4)≧(1)≧(3). 2,5-Bis(D-arabino-tetrahydroxybutyl)-2,5-dihydropyrazine (1) and 2,5-dihydro-3,6-dimethylpyrazine (2) have already been described in terms of DNA breaking activity in a previous paper.The activity was suggested to be due to the dihydropyrazine skeleton, in addition to active oxygen radicals formed in an aqueous solutoin. The introduction of a methyl group to the dihydropyrazine skeleton enhanced the activities of dihydropyrazine derivatives. The possible chemical basis for DNA strand breakage by dihydropyrazine derivatives, especially in the presence of Cu^<2+>, was discussed.

• 公益社団法人日本薬学会の論文
• 1996-10-15

著者

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• YAMAGUCHI Tadatoshi

  Faculty of Pharmaceutical Sciences, Sojo University

• KASHIGE Nobuhiro

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YAMAGUCHI Tadatoshi

  Department of Hygiene, Miyazaki Medical College

• MIAKE Fumio

  Faculty of Pharmaceutical Sciences, Fukuoka University

• WATANABE Kenji

  Faculty of Pharmaceutical Sciences, Fukuoka University

• MISHIRO Noriko

  Department of Hygiene, Miyazaki Medical College

• Yamaguchi Tokio

  Faculty Of Pharmaceutical Sciences Sojo University

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Watanabe K

  Department Of Agriculture Tokyo University Of Agriculture

• Miake F

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Miake Fumio

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Mishiro N

  Department Of Hygiene Miyazaki Medical College

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Mishiro Noriko

  Department Of Hygiene Miyazaki Medical College

• Tadatoshi Yamaguchi

  Faculty Of Hygiene Miyazaki Medical College

• Yamaguchi Tadatoshi

  Faculty Of Pharmaceutical Sciences Sojo University

• Kashige Nobuhiro

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Watanabe Kenji

  Faculty Of Fisheries Kagoshima University

• Miake Fumio

  Faculty of Pharmaceutical Science, Fukuoka University

• Kashige Nobuhiro

  Faculty of Pharmaceutical Science, Fukuoka University

• WATANABE Kenji

  Faculty of Agriculture, Gifu University

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