Superoxide Dismutase from the Silkworm, Bombyx mori : Sequence, Distribution, and Overexpression
スポンサーリンク
概要
- 論文の詳細を見る
Superoxide dismutase (SOD) is an enzyme facilitating the removal of superoxide anions from living organisms. This study focused on SOD from the silkworm, Bombyx mori (bmSOD). cDNA encoding bmSOD was amplified by reverse transcriptase–polymerase chain reaction. The deduced amino acid sequence of bmSOD indicated that the residues forming the Cu/Zn binding site are conserved and that the sequence is in 60% identity to that of the Drosophila melanogaster. B. mori SOD was also close to the D. melanogaster SOD in a phylogenetic tree. The bmSOD mRNA and the enzyme activity were widely distributed in diverse tissues. bmSOD functionally overexpressed in Escherichia coli in a soluble form was purified, and its stability was examined. bmSOD at 4 °C retained almost all of its original activity after incubation at pH 4–11 for 24 h. Incubation (pH 7) for 30 min at temperatures below 40 °C also affected activity insignificantly.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
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Yamamoto K
Kyushu Univ. Fukuoka Jpn
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Zhang Pingbo
College Of Sericulture And Biotechnology Southwest University
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Banno Y
Kyushu Univ. Fukuoka Jpn
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Banno Yutaka
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Fujii Hiroshi
Dep. Of Bioscience And Biotechnology Shinshu Univ.
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Yamamoto K
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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ASO yoichi
Laboratory of Protein Chemistry & Engineering, Kyushu University
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Yamamoto K
Kyushu Univ. Fukuoka
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Fujii Hiroshi
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Yamamoto Kohji
Laboratory Of Soil Science And Plant Nutrition College Of Agriculture:(present Address)wakayama Pref
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Yamamoto Kohji
Laboratory Of Insect Genetic Resources Division Of Applied Genetic Resources Department Of Genetic R
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MIAKE Fumio
Faculty of Pharmaceutical Sciences, Fukuoka University
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MIAKE Fumio
Microbiology Laboratory, Faculty of Phamaceutical Sciences, School of Medicine, Fukuoka University
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Fujimoto Hirofumi
Division Of Radiological Protection And Biology National Institute Of Infectious Diseases
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Miake F
Faculty Of Pharmaceutical Sciences Fukuoka University
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Aso Y
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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ZHANG Pingbo
Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
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KASHIGE Nobuhiro
Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University
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FUJII Hiroshi
Yamagata Agricultural Experimental Station, Shounai Branch
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Fujii H
Yamagata Agricultural Experimental Station Shounai Branch
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Kashige Nobuhiro
Faculty Of Pharmaceutical Sciences Fukuoka University
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Fujii Hiroshi
Interdisciplinary Graduate School Of Science And Technology Shinshu University
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ASO Yoichi
Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University
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