Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.
著者
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UJITA Minoru
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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UENO Yoshinori
Institute of Genetic Resources, Kyushu University
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BANNO Yutaka
Institute of Genetic Resources, Kyushu University
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FUJII Hiroshi
Institute of Genetic Resources, Kyushu University
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Ujita M
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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OKUMURA Hiroki
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Banno Y
Kyushu Univ. Fukuoka Jpn
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Fujii Hiroshi
Dep. Of Bioscience And Biotechnology Shinshu Univ.
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Ueno Yoshinori
Institute Of Genetic Resources Faculty Of Agriculture Kyushu University
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Ujita Minoru
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Yamanaka Mayumi
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Maeno Yumiko
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Yoshida Koki
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Ohshio Wakana
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Banno Yutaka
Institute Of Genetic Resources Faculty Of Agriculture Kyushu University Graduated School
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Fujii H
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Okumura Hiroki
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Okumura Hiroki
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Yoshida Koki
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Maeno Yumiko
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Ohshio Wakana
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Yamanaka Mayumi
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Fujii Hiroshi
Institute For Life Support Technology Yamagata Technopolis Foundation
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Maeno Yumiko
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University
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Yoshida Koki
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University
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Yamanaka Mayumi
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University
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