Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
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概要
- 論文の詳細を見る
CI-8 is the chymotrypsin inhibitor in hemolymph from the silkworm, Bombyx mori. It occurs in the midgut at the spinning stage of larva, but little information on the mechanism of its uptake in the midgut is available. We found that two polypeptides interacting with CI-8 are in the midgut membrane, and we purified them using a biotinylated CI-8, viz., p29 and p60, having molecular sizes of 29 kDa and 60 kDa respectively. The structures of p29 and p60 were examined by N-terminal amino acid sequencing and peptide mass mapping, including tryptic digestion. p29 was highly similar to the matured 19G1-30K lipoprotein from hemolymph, but p60 was novel. Purified p29 was recognized by anti-19G1-30K antibody, and was confirmed to be similar to 19G1-30K. The antibody also neutralized the CI-8 binding ability of p29 in the midgut membrane. p29 and p60 are perhaps proteinaceous factors involved in the uptake of CI-8 into the midgut through the membrane.
- 社団法人 日本農芸化学会の論文
- 2006-07-23
著者
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UJITA Minoru
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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UENO Yoshinori
Institute of Genetic Resources, Kyushu University
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BANNO Yutaka
Institute of Genetic Resources, Kyushu University
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FUJII Hiroshi
Institute of Genetic Resources, Kyushu University
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Ujita M
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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Yamamoto K
Kyushu Univ. Fukuoka Jpn
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Banno Y
Kyushu Univ. Fukuoka Jpn
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Fujii Hiroshi
Dep. Of Bioscience And Biotechnology Shinshu Univ.
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Ueno Yoshinori
Institute Of Genetic Resources Faculty Of Agriculture Kyushu University
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Yamamoto K
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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ASO yoichi
Laboratory of Protein Chemistry & Engineering, Kyushu University
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Yamamoto K
Kyushu Univ. Fukuoka
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Yamamoto Kohji
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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HE Ningjia
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
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He Ningjia
Institute Of Genetic Resources Faculty Of Agriculture Kyushu University
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Ujita Minoru
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Fujimoto Hirofumi
Division Of Radiological Protection And Biology National Institute Of Infectious Diseases
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Aso Y
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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FUJII Hiroshi
Yamagata Agricultural Experimental Station, Shounai Branch
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Banno Yutaka
Institute Of Genetic Resources Faculty Of Agriculture Kyushu University Graduated School
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Fujii H
Yamagata Agricultural Experimental Station Shounai Branch
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Fujii Hiroshi
Interdisciplinary Graduate School Of Science And Technology Shinshu University
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Fujii Hiroshi
Institute For Life Support Technology Yamagata Technopolis Foundation
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ASO Yoichi
Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University
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