Isolation and Molecular Characterization of the Locked-on Mutant of Mg^<2+> Sensor PhoQ in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
A Mg2+ sensor mutant (PhoQD179L(A)) in which D179 of PhoQ was changed into L or A was isolated and characterized in Escherichia coli. PhoQ–PhoP regulon genes, phoPQ, mgtA and mgrB transcriptions were repressed at a high Mg2+ concentration in WQ3007 (phoQ-defective strain)/pHO119, but not in WQ3007/pHO179L(A). The in vitro autophosphorylation activity of membrane-bound PhoQ was repressed by Mg2+ (10 mM), but that of membrane-bound PhoQD179L(A) was not. Furthermore, the phosphotransfer from membrane-bound PhoQ to PhoP was also repressed by Mg2+, but was not observed in membrane-bound PhoQD179L(A). These results suggest that PhoQD179L(A) is a locked-on mutant that is defective in extracellular Mg2+-sensing and that the D179 amino acid residue of PhoQ plays an essential role in signal transfer between the Mg2+-sensory and histidine kinase domain of PhoQ.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
-
Yamamoto K
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
-
Yamamoto K
Kyushu Univ. Fukuoka
-
Utsumi Ryutaro
Department of Agricultural Chemistry, Kinki University
-
YAMAMOTO Kaneyoshi
Department of Agricultural Chemistry, Kinki University
-
MINAGAWA Shu
Department of Agricultural Chemistry, Kinki University
-
Minagawa Shu
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
-
Utsumi R
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
-
Utsumi Ryutaro
Department Of Advanced Bioscience Kinki University
-
OKURA Ryouta
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
TSUCHITANI Hiroki
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
HIRAO Kiyo
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
Hirao Kiyo
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
-
Okura Ryouta
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
-
Minagawa Shu
Department Of Advanced Bioscience Kinki University
-
Tsuchitani Hiroki
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
関連論文
- CHARACTERIZATION OF THE SIGNAL TRANSDUCTION VIA EVGS AND EVGA IN ESCHERICHIA COLI
- Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea : Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori
- Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
- X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
- Antibacterial Agents that Inhibit Histidine Protein Kinase YycG of Bacillus subtilis(Biochemistry & Molecular Biology)
- Chemistry of Zerumbone. 2. Regulation of Ring Bond Cleavage and Unique Antibacterial Activities of Zerumbone Derivatives(Organic Chemistry)
- SELECTIVE DESTRUCTION OF ACTIN-BASED CYTOSKELETON NETWORKS IN THE IMMATURE STARFISH OOCYTE BY THEONELLAPEPTOLIDE IE, A PEPTIDE FROM A MARINE SPONGE(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society
- Malformation of Immature Starfish Oocytes by Theonellapeptolide Ie, a Tridecapeptide Lactone from a Marine Sponge Petrosia Species, through Disturbance of Cortical F-Actin Distribution(Biochemistry & Molecular Biology)
- X-Ray Study of Calcium Phosphates Formed in the Presence of Citrate
- Inhibitory Effect of Phosphorylated Oligosaccharides Prepared from Potato Starch on the Formation of Calcium Phosphate
- Effective Production of Glycosyl-steviosides by α-1,6 Transglucosylation of Dextrin Dextranase
- Classification of Some α-Glucosidases and α-Xylosidases on the Basis of Substrate Specificity
- Substrate Specificity of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Transfer Action of α-D-Xylosidases from Aspergillus flavus MO-5 on p-Nitrophenyl-α-D-xylopyranoside
- Effective Dextran Production from Starch by Dextrin Dextranase with Debranching Enzyme
- Structure of Dextran Synthesized by Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Purification and Characterization of an Intracellular α-D-Xylosidase II from Aspergillus flavus MO-5
- Isolation of Aspergillus flavus MO-5 Producing Two Types of Intracellular α-D-Xylosidases : Purification and Characterization of α-D-Xylosidase I
- Dextran Synthesis from Reduced Maltooligosaccharides by Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Detailed Action Mechanism of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Purification and Some Properties of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- Identification and Characterization of a Potent Antibacterial Agent, NH125 against Drug-resistant Bacteria
- Inhibitory Activity of Alginates against the Formation of Calcium Phosphate
- Mutational Analysis of the fic Promoter Recognized by RpoS (σ^) in Escherichia coli
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate (2):Importance of the P2 and P4 Residues
- Trial for linkage mapping of phenotypic characters and EST cDNA clones in the silkworm, Bombyx mori
- Genes Encoding Small Heat Shock Proteins of the Silkworm, Bombyx mori
- Proteomic Studies of Lipopolysaccharide-induced Polypeptides in the Silkworm, Bombyx mori
- In Vivo and in Vitro Interactions of the Bombyx mori Chymotrypsin Inhibitor b1 with Escherichia coli
- Inheritance of a Body Shape Mutant Named "Short Segment" of the Domesticated Silkworm, Bombyx mori
- Overproduction and characterization of hydroxyphenylalanine oxidoreductase of the silkmoth
- Physicochemical Study of Calcium-binding Properties of Chemical Substances as Inhibitors against Calcium Phosphate Insolubilization
- Inhibitory Activity of Oligo- and Poly-L-glutamic Acids against Calcium Phosphate Insolubilization and Calcium Binding with Special Relevance to Their Molecular Weight Dependence
- Superoxide Dismutase from the Silkworm, Bombyx mori : Sequence, Distribution, and Overexpression
- Utility of Dry Gel from Two-dimensional Electrophoresis for Peptide Mass Fingerprinting Analysis of Silkworm Proteins
- Genetic Analysis of Basic Chymotrypsin Inhibitors in the Hemolymph of the Silkworm, Bombyx mori
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate
- Mutational Analysis of the Putative Substrate-binding Site of 3C Proteinase of Coxsackievirus B3
- Expression and Purification of Recombinant 3C Proteinase of Coxsackievirus B3
- Nucleotide Sequence of the Glycerol-3-phosphate Dehydrogenase Gene of Escherichia coli and Regulation by the cAMP-CRP Complex(Biological Chemistry)
- Integration of the twenty-fourth and twenty-seventh linkage groups of the silkwom, Bombyx mori
- Investigation of Integration of Linkage Mapping of Phenotypic Characters and EST cDNA Clones in the Silkworm, Bombyx mori
- Expression and Characterization of a Sigma-Class Glutathione S-Transferase of the Fall Webworm, Hyphantria cunea
- Chymotrypsin and trypsin inhibitors of the saturniid moth, Caligula japonica (Lepidoptera, Saturniidae)
- Purification and characterization of novel Kunitz-type chymotrypsin inhibitor of the silkworm, Bombyx mori
- Basic information for the use of as an experimental animal
- Proteomic Studies of Isoforms of the P25 Component of Bombyx mori Fibroin
- Identification and Overexpression of Y-box Protein Isoform of the Silkworm, Bombyx mori
- Isolation and Molecular Characterization of the Locked-on Mutant of Mg^ Sensor PhoQ in Escherichia coli
- Ascochytatin, a Novel Bioactive Spirodioxynaphthalene Metabolite Produced by the Marine-derived Fungus, Ascochyta sp. NGB4
- Binding of the Protein from Thermus aquaticus ISLtaq1 to Its Inverted Repeat in Vitro
- Identification of the Promoter Region and the Transcriptional Regulatory Sequence of the evgAS Operon of Escherichia coli
- Molecular Interaction between Proteins Involved in EvgAS Signal Transduction of Escherichia coli
- Cysteine Uptake for Accumulation of Glutathione by the Cyanobacterium Synechocystis strain PCC 6803
- Structural Properties of Silkworm Small Heat-Shock Proteins : sHSP19.9 and sHSP20.8
- X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
- Effect of Intracellular Glutathione on Heat-induced Cell Death in the Cyanobacterium, Synechocystis PCC 6803
- Essential Role of Phosphorylation in Signal Transduction by a Chimeric Receptor Composed of the Chemoreceptor Tar and Osmosensor EnvZ(Biological Chemistry)
- GROWTH REGULATION BY cAMP IN ESCHERICHIA COLI
- Isolation and Characterization of Inhibitors of the Essential Histidine Kinase, YycG in Bacillus subtilis and Staphylococcus aureus
- Isolation and Characterization of the IS3-like Element from Thermus aquaticus
- Nucleotide Sequence of the Uricase Gene from Bacillus sp. TB-90^1
- Cloning of the Creatinine Amidohydrolase Gene from Pseudomonas sp. PS-7
- Transcriptional regulation of the Na^+-NADH : quinone oxidoreductase gene, nqr, in Vibrio anguillarum, a fish pathogen, in the stationary phase
- Molecular Cloning and Characterization of the Fusaric Acid-resistance Gene from Pseudomonas cepacia(Biological Chemistry)
- Isolation and Characterization of the Heat-responsive Genes in Escherichia coli
- Effects of cya and cid Mutations on Cell Growth in Escherichia coli(Biological Chemistry)
- Expression and Characterization of a Sigma-Class Glutathione S-Transferase of the Fall Webworm, Hyphantria cunea
- Biosynthesis of Bisorbicillinoid in Trichoderma sp. USF-2690 ; Evidence for the Biosynthetic Pathway, via Sorbicillinol, of Sorbicillin, Bisorbicillinol, Bisorbibutenolide, and Bisorbicillinolide(Organic Chemistry)
- Novel Fungal Metabolites, Demethylsorbicillin and Oxosorbicillinol, Isolated from Trichoderma sp. USF-2690
- Transcription of emrKY is Regulated by the EvgA-EvgS Two-Component System in Escherichia coli K-12
- Characterization of CitA-CitB Signal Transduction Activating Genes Involved in Anaerobic Citrate Catabolism in Escherichia coli
- Anaerobic Regulation of Citrate Fermentation by CitAB in Escherichia coli
- Two Modes of Autoregulation of the murR Repressor in Escherichia coli
- Bacterial Signal Transduction Systems for Environmental Adaptation and Their Inhibitors
- Characterization of Copper-Inducible Promoters Regulated by CpxA/CpxR in Escherichia coli
- Molecular Mechanism of Transcriptional Cascade Initiated by the EvgS/EvgA System in Escherichia coli K-12
- Degradation of interleukin 8 by the serine protease MucD of Pseudomonas aeruginosa
- Walkmycin B targets WalK (YycG), a histidine kinase essential for bacterial cell growth
- Novel antibacterial compounds specifically targeting the essential WalR response regulator
- Induction of YdeO, a Regulator for Acid Resistance Genes, by Ultraviolet Irradiation in Escherichia coli
- Complementation of the exoS gene in the pvdE pyoverdine synthesis gene-deficient mutant of Pseudomonas aeruginosa results in recovery of the pvdE gene-mediated penetration through the intestinal epithelial cell barrier but not the pvdE-mediated virulence
- Mechanism of Activation of PhoQ/PhoP Two-Component Signal Transduction by SafA, an Auxiliary Protein of PhoQ Histidine Kinase in Escherichia coli
- Type IV pilus protein PilA of Pseudomonas aeruginosa modulates calcium signaling through binding the calcium-modulating cyclophilin ligand