Expression of Prophenoloxidase mRNA during Silkworm Hemocyte Development
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概要
- 論文の詳細を見る
Two clones encoding different prophenoloxidase isoforms were amplified by polymerase chain reaction of RNA from the hemocytes of an experimental strain of Bombyx mori. The nucleotide sequences of the clones and the deduced amino acid sequences were confirmed to be nearly identical to those of the orthologous clones previously obtained from a commercial race of B.mori. Northern blot hybridization using these clones as probes demonstrated that the prophenoloxidase mRNA in the hemocytes is expressed in a stage-specific manner during the final larval instar and pupal stage, showing a peak one day before pupation in males and on the day of pupation in females. A sexual difference was also observed when the content of prophenoloxidase protein in the hemolymph (including hemocytes) was measured by an enzyme-linked immunosorbent assay.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
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Yamamoto K
Kyushu Univ. Fukuoka Jpn
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Koga K
Laboratory Of Silkworm Sciences Faculty Of Agriculture Kyushu University Graduate School
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Koga K
Faculty Of Agriculture Kyushu University Graduate School:(present Office)faculty Of Engineering Kyus
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Koga Katsumi
Laboratory Of Sericultural Chemistry Faculty Of Agriculture Kyushu University
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Koga Katsumi
Laboratory Of Sericultural Chemistry Agricultural Chemistry Institute Kyushu University
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Fujii Hiroshi
Dep. Of Bioscience And Biotechnology Shinshu Univ.
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ASO yoichi
Laboratory of Protein Chemistry & Engineering, Kyushu University
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Kusakabe Takahiro
Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmenta
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Fujii Hiroshi
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Yamamoto Kohji
Laboratory Of Soil Science And Plant Nutrition College Of Agriculture:(present Address)wakayama Pref
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Yamamoto Kohji
Laboratory Of Insect Genetic Resources Division Of Applied Genetic Resources Department Of Genetic R
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YAKIYAMA Masatoshi
Laboratory of Insect Genetic Resources, Kyushu University Graduate School of Bioresource and Bioenvi
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ISHIGURO Masatsune
Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an
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Aso Y
Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U
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Ishiguro M
Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University
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Kusakabe Takahiro
Laboratory Of Silkworm Science Kyushu University Graduate School Of Bioresource And Bioenvironmental
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Kusakabe Takahiro
Laboratory Of Silkworm Science Faculty Of Agriculture Graduate School Kyushu University
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Ishiguro M
Kyushu Univ. Fukuoka Jpn
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Ishiguro Masatsune
Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr
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Yakiyama Masatoshi
Laboratory Of Insect Genetic Resources Kyushu University Graduate School Of Bioresource And Bioenvir
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Fujii H
Yamagata Agricultural Experimental Station Shounai Branch
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Fujii Hiroshi
Interdisciplinary Graduate School Of Science And Technology Shinshu University
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Ishiguro Masatsune
Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
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ASO Yoichi
Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University
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